渗透调节物质及有机附加物对小麦体细胞胚发生影响的研究(英文)

文献类型： 中文期刊

第一作者： 孙朝霞

作者： 孙朝霞;侯思宇;王玉国

作者机构：

期刊名称： Agricultural Science & Technology

ISSN： 1009-4229

年卷期： 2008 年 03 期

页码： 52-54

摘要： [Objective] The study aimed to reveal the effect of osmosis-regulating substances and organic appendices on somatic embryogenesis in wheat. [Method] The suitable concentration combination of appendices was optimized by adding different concentrations of osmosis-regulating substances including mannitol, sorbitol and organic appendices such as Gln, CH and LH, into the somatic embryogenesis in wheat. [Result] The mannitol or sorbitol lower than 40 g/L was helpful for improving somatic embryogenesis; there was no significant difference in the induction rate of somatic embryogenesis when 300-500 mg/L Gln、CH or LH was respectively added into the induced medium, while somatic embryogenesis could be enhanced dramatically in the presence of 500 mg/L Gln together with 300 mg/L CH. [Conclusion] Somatic embryogenesis could be improved to some extent by different concentrations of osmosis-regulating substances and organic appendices, which laid foundation for establishing a more perfect system of somatic embryogenesis in wheat.

分类号： S512.1

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