Identification of characteristic markers for monofloral honey of Astragalus membranaceus var. mongholicus Hsiao: A combined untargeted and targeted MS-based study
文献类型： 外文期刊
第一作者： Zhao, Tong
作者： Zhao, Tong;Zhao, Liuwei;Wang, Miao;Qi, Suzhen;Xue, Xiaofeng;Wu, Liming;Li, Qiangqiang
作者机构：
关键词： Monofloral honey; Untargeted; targeted mass spectrometry; Characteristic markers; Antioxidant activity; Astragalus membranaceus var; mongholicus Hsiao
期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）
ISSN： 0308-8146
年卷期： 2023 年 404 卷
页码：
收录情况： SCI
摘要： Astragalus membranaceus var. mongholicus Hsiao (Am) is a widely used traditional Chinese herbal medicine. The monofloral honey from Am plant nectar collected by honeybees (MH-Am) has potential medicinal activities. Quality control of MH-Am requires discovery of characteristic markers. In this study, calycosin and formononetin were identified as reliable chemical markers for MH-Am authentication, which were shared with its plant (P-Am), but absent in other honeys based on untargeted mass spectrometry (MS) analysis. The contents of calycosin and formononetin in MH-Am, other honeys and P-Am were determined through a targeted MS-based quantitative approach. Furthermore, free radical scavenging assays showed that calycosin functioned directly in the antioxidative activity of MH-Am. Thus, calycosin has great potential to be certified as a bioactive marker contributing to future quality control of commercial MH-Am products.
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