Characterization of sexual maturity-associated N6-methyladenosine in boar testes

文献类型： 外文期刊

第一作者： Zhang, Pengfei

作者： Zhang, Pengfei;Zhang, Fei;Sui, Heming;Yang, Xingyu;Ji, Yiming;Zheng, Shenghao;Li, Wei;Cheng, Kun;Zhang, Xiaodong;Cao, Zubing;Zhang, Yunhai;Sui, Heming;Wang, Chonglong;Jiao, Jun

作者机构：

关键词： Pig; m6A; Testicular development; Fertility

期刊名称：BMC GENOMICS （ 影响因子：4.4； 五年影响因子：4.7 ）

ISSN： 1471-2164

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： Background The health and size of the testes are crucial for boar fertility. Testicular development is tightly regulated by epigenetics. N6-methyladenosine (m6A) modification is a prevalent internal modification on mRNA and plays an important role in development. The mRNA m6A methylation in boar testicular development still needs to be investigated.Results Using the MeRIP-seq technique, we identify and profile m6A modification in boar testes between piglets and adults. The results showed 7783 distinct m6A peaks in piglets and 6590 distinct m6A peaks in adults, with 2,471 peaks shared between the two groups. Enrichment of GO and KEGG analysis reveal dynamic m6A methylation in various biological processes and signalling pathways. Meanwhile, we conjointly analyzed differentially methylated and expressed genes in boar testes before and after sexual maturity, and reproductive related genes (TLE4, TSSK3, TSSK6, C11ORF94, PATZ1, PHLPP1 and PAQR7) were identified. Functional enrichment analysis showed that differential genes are associated with important biological functions, including regulation of growth and development, regulation of metabolic processes and protein catabolic processes.Conclusion The results demonstrate that m6A methylation, differential expression and the related signalling pathways are crucial for boar testicular development. These results suggest a role for m6A modification in boar testicular development and provided a resource for future studies on m6A function in boar testicular development.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序