Material-to-Application Integration: Rapid Fabrication of Field-Deployable Hydrogel-SiO2 DNA Separator for Low-Resource Point-of-Care Diagnostics

文献类型： 外文期刊

第一作者： Li, Peipei

作者： Li, Peipei;Li, Xinrong;Wu, Haojie;Yue, Dongmei;Shang, Yubo;Gao, Shan;Wang, Bai;Jiang, Jingwei;Zhou, Zunchun;Li, Xinrong;Wu, Haojie;Jiang, Xiaobin;Gao, Shan;Wang, Bai;Jiang, Jingwei;Zhou, Zunchun

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关键词： hydrogel fabrication; nucleic acid extraction; point-of-care detection; resource-limited settings; surface modification

期刊名称：ADVANCED SCIENCE （ 影响因子：14.1； 五年影响因子：15.6 ）

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年卷期： 2025 年

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收录情况： SCI

摘要： Current nucleic acid (NA) diagnostics are hindered in resource-limited settings by equipment needs and high costs. S(PAA-SiO2) is developed, a hydrogel-SiO2 composite deoxyribonucleic acid (DNA) separator addressing these challenges through rapid (<10 min), simple, and skill-free preparation, ultralow cost ($0.028 per unit), and equipment-minimized mass production capacity (96 units per batch). Further systematic investigation of SiO2 particle surface modification revealed critical enhancements in DNA adsorption properties. With maintaining structural thermal stability, SiO2 integration significantly improved the surface roughness, specific surface area, and hydrophobicity, leading to hydrophobic and salt bridge effect-enhanced DNA adsorption. Especially, SiO2 modification with mixed particle size formed a hierarchical microstructure to promote turbulence and interface interaction. Device S(PAA-SiO2-Mix) achieved a higher DNA extraction yield than the commercial kits. It possesses potential value for Polymerase Chain Reaction (PCR) diagnosis of bacterial, viral, parasitic, and fungal pathogens. By orchestrating three technological progresses - rapid S(PAA-SiO2-Mix) fabrication, high-throughput DNA extraction, and visual loop-mediated isothermal amplification (LAMP) - a field-deployable diagnostic workflow is established for Vibrio parahaemolyticus (v. parahaemolyticus, Vpa). This platform achieves equipment-minimized point-of-care detection in <40 min with a sensitivity of 10 CFU mL(-1). Its simplicity, speed, and accuracy offer a transformative solution for resource-limited diagnostics. This work advances separation interface design and presents a novel vision for integrated, equipment-free molecular detection systems.

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