Design and Application of Biosafe Coronavirus Engineering Systems without Virulence

文献类型： 外文期刊

第一作者： Wu, Guoqiang

作者： Wu, Guoqiang;Li, Qiaoyu;Dai, Junbiao;Mao, Guobin;Ma, Yingxin;Wu, Guoqiang;Li, Qiaoyu;Dai, Junbiao;Mao, Guobin;Ma, Yingxin;Wu, Guoqiang;Li, Qiaoyu;Dai, Junbiao

作者机构：

关键词： coronavirus; biosafety; pseudovirus; trans-complementation system; application

期刊名称：VIRUSES-BASEL （ 影响因子：4.7； 五年影响因子：4.8 ）

ISSN：

年卷期： 2024 年 16 卷 5 期

页码：

收录情况： SCI

摘要： In the last twenty years, three deadly zoonotic coronaviruses (CoVs)-namely, severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and SARS-CoV-2-have emerged. They are considered highly pathogenic for humans, particularly SARS-CoV-2, which caused the 2019 CoV disease pandemic (COVID-19), endangering the lives and health of people globally and causing unpredictable economic losses. Experiments on wild-type viruses require biosafety level 3 or 4 laboratories (BSL-3 or BSL-4), which significantly hinders basic virological research. Therefore, the development of various biosafe CoV systems without virulence is urgently needed to meet the requirements of different research fields, such as antiviral and vaccine evaluation. This review aimed to comprehensively summarize the biosafety of CoV engineering systems. These systems combine virological foundations with synthetic genomics techniques, enabling the development of efficient tools for attenuated or non-virulent vaccines, the screening of antiviral drugs, and the investigation of the pathogenic mechanisms of novel microorganisms.

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