Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae

文献类型： 外文期刊

第一作者： Cheng, Li

作者： Cheng, Li;Zhao, Shijun;Li, Tianyi;Hou, Sha;Luo, Zhouqing;Yu, Wenfei;Jiang, Shuangying;Ma, Yingxin;Cai, Yizhi;Dai, Junbiao;Zhao, Shijun;Yu, Wenfei;Dai, Junbiao;Luo, Zhouqing;Xu, Jinsheng;Monti, Marco;Schindler, Daniel;Cai, Yizhi;Zhang, Weimin;Boeke, Jef D.;Zhang, Weimin;Boeke, Jef D.;Hou, Chunhui;Boeke, Jef D.;Dai, Junbiao;Dai, Junbiao;Li, Tianyi

作者机构：

期刊名称：NATURE COMMUNICATIONS （ 影响因子：16.6； 五年影响因子：17.0 ）

ISSN：

年卷期： 2024 年 15 卷 1 期

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收录情况： SCI

摘要： Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) is a promising tool to study genomic rearrangements. However, the potential of SCRaMbLE to study genomic rearrangements is currently hindered, because a strain containing all 16 synthetic chromosomes is not yet available. Here, we construct SparLox83R, a yeast strain containing 83 loxPsym sites distributed across all 16 chromosomes. SCRaMbLE of SparLox83R produces versatile genome-wide genomic rearrangements, including inter-chromosomal events. Moreover, when combined with synthetic chromosomes, SCRaMbLE of hetero-diploids with SparLox83R leads to increased diversity of genomic rearrangements and relatively faster evolution of traits compared to hetero-diploids only with wild-type chromosomes. Analysis of the SCRaMbLEd strain with increased tolerance to nocodazole demonstrates that genomic rearrangements can perturb the transcriptome and 3D genome structure and consequently impact phenotypes. In summary, a genome with sparsely distributed loxPsym sites can serve as a powerful tool for studying the consequence of genomic rearrangements and accelerating strain engineering in Saccharomyces cerevisiae.

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