Determination of jasmonic acid in bark extracts from Hevea brasiliensis by capillary electrophoresis with laser-induced fluorescence detection

文献类型： 外文期刊

第一作者： Zhang, ZL

作者： Zhang, ZL;Liu, X;Li, DF;Lu, YT

作者机构：

关键词： derivatization;jasmonic acid;5-bromomethylfluorescein;capillary electrophoresis laser induced fluorescent detection;PERFORMANCE LIQUID-CHROMATOGRAPHY;IONIZATION-MASS-SPECTROMETRY;CARBOXYL-CONTAINING ANALYTES;ABSCISIC-ACID;METHYL JASMONATE;QUANTIFICATION;PLANTS;DERIVATIZATION;BIOSYNTHESIS;LEAVES

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A simple and sensitive method is described for determination of jasmonic acid (JA) in plant tissues. The method is based on derivatization of JA with 5-bromomethylfluorescein (5-BMF) and separation and quantification of the resulting 5-BMF-JA derivative by capillary electrophoresis coupled to laser-induced fluorescence detection (CE-LIF). The derivatization conditions were studied in detail. Our results indicated that 5-BMF-labeled JA could be well separated from other plant hormones present in the sample by use of 20 mmol L-1 borate buffer (pH 8.5). The response to JA was a linear function of concentration in the range 1 to 100 mu mol L-1, with a correlation of 0.9986. Our preliminary work showed that the proposed method had fairly good selectivity and sensitivity. Only small amounts of plant sample are needed to complete the analysis. This described method enables the analysis of JA in crude extracts without extra purification and enrichment procedures.

分类号： O65

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