Expression of biologically active recombinant equine interferon-gamma by two different baculovirus gene expression systems using insect cells and silkworm larvae

文献类型： 外文期刊

第一作者： Wu, DL

作者： Wu, DL;Murakami, K;Liu, NH;Inoshima, Y;Yokoyama, T;Kokuho, T;Inumaru, S;Matsumura, T;Kondo, T;Nakano, K;Sentsui, H

作者机构：

关键词： baculovirus;equine;recombinant interferon-gamma;silkworm

期刊名称：CYTOKINE （ 影响因子：3.861； 五年影响因子：4.257 ）

ISSN： 1043-4666

年卷期： 2002 年 20 卷 2 期

页码：

收录情况： SCI

摘要： The full-length equine interferon-gamma (eIFN-gamma) cDNA, including the secretion signal peptide coding region, was recloned into baculovirus transfer vector pAcYM1. This vector was co-transfected with Autographa californica nuclear polyhedrosis virus DNA or hybrid nuclear polyhedrosis virus DNA into Spodoptera frugiperda cells. The recombinant viruses, named AcEIFN-gamma and HyEIFN-gamma, were then recovered. Recombinant eIFN-gamma (relFN-gamma) was accumulated in the culture fluid of the AcEIFN-gamma or HyEIFN-gamma infected Tricoplusia ni-derived cell line, BTI TN 5BI-4, and hemolymph of HyEIFN-gamma infected silkworm larvae. These re1FN-gamma forms were shown to be 14, 16, 18 and 20 kDa proteins, and glycosylated as confirmed by SDS-PAGE and tunicamycin treatment. Both reIFN-gamma proteins, showed high-level biological activities to vesicular stomatitis virus by cytopathic effect reduction assay, and MHC class II antigen induction on the equine fetal kidney-78 cell line. (C) 2002 Elsevier Science Ltd. All rights reserved.

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