Expression of equine interleukin-18 by baculovirus expression system and its biologic activity

文献类型: 外文期刊

第一作者: Wu, DL

作者: Wu, DL;Murakami, K;Liu, NH;Konishi, M;Muneta, Y;Inumaru, S;Kokuho, T;Sentsui, H

作者机构:

关键词: baculovirus;cytokine;equine;interleukin-18

期刊名称:MICROBIOLOGY AND IMMUNOLOGY ( 影响因子:1.955; 五年影响因子:1.917 )

ISSN: 0385-5600

年卷期: 2004 年 48 卷 6 期

页码:

收录情况: SCI

摘要: The equine interleukin-18 (IL-18) cDNA that contains the coding sequence was cloned and a recombinant baculovirus, named AcEIL-18, was constructed. The recombinant protein of the equine IL-18 was expressed by AcEIL-18 and its expression was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Insect cells infected with AcEIL-18 secreted a precursor IL-18 with 24 kilo dalton (kDa) into the culture supernatant. Western blot analysis showed that mature equine IL-18 about 18 kDa was also confirmed without co-expression of caspase-1. Culture supernatant from AcEIL-18 infected cells showed a synergistic effect with recombinant human Interleukin-12 for induction of interferon-gamma gene expression in equine peripheral mononuclear cells, indicating that the recombinant equine IL-18 expressed in this study also has biological activity without any treatment.

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