Insights into chromosomal evolution and sex determination of Pseudobagrus ussuriensis (Bagridae, Siluriformes) based on a chromosome-level genome

文献类型： 外文期刊

第一作者： Zhu, Chuankun

作者： Zhu, Chuankun;Pan, Zhengjun;Wang, Hui;Chang, Guoliang;Wu, Nan;Ding, Huaiyu;Zhao, Haitao;Liu, Haiyang;Cheng, Lei;Sun, Yanhong;Zhang, Lei;Yu, Xiangsheng

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关键词： Pseudobagrus ussuriensis; whole-genome sequencing; chromosome evolution; sex-determining region; sex chromosome

期刊名称：DNA RESEARCH （ 影响因子：4.477； 五年影响因子：5.358 ）

ISSN： 1340-2838

年卷期： 2022 年 29 卷 4 期

页码：

收录情况： SCI

摘要： Pseudobagrus ussuriensis is an aquaculture catfish with significant sexual dimorphism. In this study, a chromosome-level genome with a size of 741.97 Mb was assembled for female P. ussuriensis. A total of 26 chromosome-level contigs covering 97.34% of the whole-genome assembly were obtained with an N50 of 28.53 Mb and an L50 of 11. A total of 24,075 protein-coding genes were identified, with 91.54% (22,039) genes being functionally annotated. Based on the genome assembly, four chromosome evolution clusters of catfishes were identified and the formation process of P. ussuriensis chromosomes was predicted. A total of 55 sex-related quantitative trait loci (QTLs) with a phenotypic variance explained value of 100% were located on chromosome 8 (chr08). The QTLs and other previously identified sex-specific markers were located in a sex-determining region of 16.83 Mb (from 6.90 to 23.73 Mb) on chr08, which was predicted as the X chromosome. The sex-determining region comprised 554 genes, with 135 of which being differently expressed between males and females/pseudofemales, and 16 candidate sex-determining genes were screened out. The results of this study provided a useful chromosome-level genome for genetic, genomic and evolutionary studies of P. ussuriensis, and also be useful for further studies on sex-determination mechanism analysis and sex-control breeding of this fish.

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