In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus

文献类型： 外文期刊

第一作者： Liu, Panrao

作者： Liu, Panrao;Gong, Yabin;Qiu, Xusheng;Sun, Yingjie;Tan, Lei;Song, Cuiping;Liu, Weiwei;Liao, Ying;Meng, Chunchun;Ding, Chan;Yin, Yuncong;Ding, Chan

作者机构：

关键词： virulent Newcastle disease virus; metabolomic analysis; in vitro and in vivo; UHPLC-QTOF-MS

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN：

年卷期： 2019 年 11 卷 10 期

页码：

收录情况： SCI

摘要： Newcastle disease (ND) is an acute, febrile, highly contagious disease caused by the virulent Newcastle disease virus (vNDV). The disease causes serious economic losses to the poultry industry. However, the metabolic changes caused by vNDV infection remain unclear. The objective of this study was to determine the metabolomic profiling after infection with vNDV. DF-1 cells infected with the vNDV strain Herts/33 and the lungs from Herts/33-infected specific pathogen-free (SPF) chickens were analyzed via ultra-high-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) in combination with multivariate statistical analysis. A total of 305 metabolites were found to have changed significantly after Herts/33 infection, and most of them belong to the amino acid and nucleotide metabolic pathway. It is suggested that the increased pools of amino acids and nucleotides may benefit viral protein synthesis and genome amplification to promote NDV infection. Similar results were also confirmed in vivo. Identification of these metabolites will provide information to further understand the mechanism of vNDV replication and pathogenesis.

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