High efficient chemiluminescent immunoassays for the detection of diclazuril in chicken muscle based on biotin-streptavidin system
文献类型： 外文期刊
第一作者： Li, Yuan
作者： Li, Yuan;Wang, Yahui;Li, PeiPei;Yu, Wenbo;Wen, Kai;Ding, Shuangyang;Wang, Zhanhui;Yu, Xuezhi;Li, Yuan;Wang, Yahui;Li, PeiPei;Yu, Wenbo;Wen, Kai;Ding, Shuangyang;Wang, Zhanhui;Yu, Xuezhi;Wang, Yahui;Ke, Yuebin;Zhang, Yingjie
作者机构：
关键词： Chemiluminescent enzyme immunoassays; diclazuril; biotin-streptavidin system; chicken
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）
ISSN： 0954-0105
年卷期： 2020 年 31 卷 1 期
页码：
收录情况： SCI
摘要： A simple, sensitive and specific chemiluminescence enzyme immunoassay based on biotin-streptavidin system (BASA-CLEIA) for diclazuril was established, which was about 2 and 4 times more sensitive than the traditional chemiluminescence enzyme immunoassay (CLEIA) and enzyme-linked immunosorbent assay. In the optimized generic assay, the half maximal inhibitory concentration (IC50) of the BASA-CLEIA for diclazuril was 0.48 mu g/kg. The limit of detection for diclazuril was 0.02 mu g/kg. This method showed no cross reactivity (<0.01%) with toltrazuril, robenidine hydrochloride, nicarbazin, halofuginone, amprolium, monensin, and maduramycin. In chicken muscles, the average recoveries ranged from 90.3% to 120.4%. A further comparison between the developed BSAS-CLEIA and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for real samples showed good correlation (p < 0.01). The results indicated that the simple, sensitive and specific BASA-CLEIA method could be used for the analysis of diclazuril in real samples.
分类号：
- 相关文献
作者其他论文 更多>>
-
A novel study of brain microvascular endothelial cells induced by astrocyte conditioned medium for constructing blood brain barrier model in vitro: A promising tool for meningitis of teleost
作者：Chen, Yanghui;Luo, Junliang;Li, Zixin;Huang, Yu;Cai, Jia;Jiang, Dongneng;Jian, Jichang;Wang, Bei;Chen, Yanghui;Luo, Junliang;Li, Zixin;Huang, Yu;Cai, Jia;Jiang, Dongneng;Jian, Jichang;Wang, Bei;Li, Yuan;Zhang, Defeng;Qiang, Jun
关键词：Nile tilapia; Endothelial cell line; BBB; Astrocyte-conditioned medium (ACM)
-
Early detection of rubber tree powdery mildew using UAV-based hyperspectral imagery and deep learning
作者：Zeng, Tiwei;Li, Yuan;Fu, Wei;Wang, Juan;Zhang, Xirui;Zeng, Tiwei;Wang, Yong;Yang, Yuqi;Liang, Qifu;Li, Yuan;Zhang, Huiming;Fu, Wei;Wang, Juan;Zhang, Xirui;Fang, Jihua;Li, Yuan;Fang, Jihua;Li, Yuan
关键词：Powdery mildew of rubber tree; Unmanned aerial vehicle; Hyperspectral imaging; Deep learning; Different spatial resolutions; Feature selection
-
Generation of a highly specific recombinant full-length antibody for detecting ethirimol in fruit and environmental water
作者：Lei, Xingmei;Li, Peipei;Zhao, Jing;Xu, Lingyuan;Gao, Song;Zhao, Yun;She, Yongxin;Jin, Fen;Wang, Jing;Zheng, Lufei;Jin, Maojun;El-Aty, A. M. Abd;El-Aty, A. M. Abd;Li, Jia;Hammock, Bruce D.;Hammock, Bruce D.;Jin, Maojun
关键词：Ethirimol; Monoclonal antibody; Recombinant full-length antibody; Recognition mechanism; Ic-ELISA
-
The nanozymes of protein nanotubes-constructed microspheres with dual peroxidase- and catalase-like properties for M1-to-M2 macrophages repolarization and the synergistic anti-rheumatoid arthritis effect with loaded capsaicin
作者：Hou, Guohua;Chen, Shanan;Wang, Xiaoyu;Liu, Bin;Li, Xing;Li, Yuan;Hou, Guohua;Miao, Song;Pang, Jie;Zhang, Longtao;Ngai, To;Miao, Song;Hu, Wei
关键词：alpha-lactalbumin nanotube; Microsphere; Macrophage polarization; Capsaicin; Delivery system; Rheumatoid arthritis
-
The Identification of Kabatiella zeae as a Causal Agent of Northern Anthracnose of Sorghum in China and Estimation of Host Resistance
作者：Yu, Wenbo;Wang, Yu;Liu, Chunjuan;Shi, Xiaolong;Liu, Chang;Zhou, Yufei;Hu, Lan;Xu, Jing;Yan, Jichen;Jiang, Yu;Cao, Peng
关键词：sorghum northern anthracnose; Kabatiella zeae; resistance evaluation; screening of fungicides
-
The Main Compounds of Bio-Fumigant Plants and Their Role in Controlling the Root-Knot Nematode Meloidogyne incognita (Kofoid and White) Chitwood
作者：Parsiaaref, Shiva;Cao, Aocheng;Li, Yuan;Wang, Qiuxia;Yan, Dongdong;Fang, Wensheng;Huang, Bin;Zhang, Min;Cao, Aocheng;Li, Yuan;Wang, Qiuxia;Yan, Dongdong;Fang, Wensheng;Zhang, Min;Ebadollahi, Asgar;Parmoon, Ghasem
关键词：Solanum lycopersicum L.; Meloidogyne incognita; alpha-pinene; d-limonene; methylene chloride; linalool; nonanal
-
The potential for reducing aflatoxin B1 contamination of stored peanuts by soil disinfection
作者：Guo, Anmin;Zhang, Yi;Ji, Yutong;Chen, Xinhua;Zhang, Wei;Liu, Xuemei;Yan, Dongdong;Fang, Wensheng;Li, Yuan;Cao, Aocheng;Wang, Qiuxia
关键词：Fumigation; A. flavus; AFB1; Peanuts; Health hazard
