Devitalization of the immune mapped protein 1 undermines the intracellular proliferation of Toxoplasma gondii

文献类型： 外文期刊

第一作者： Li, Zhong-Yuan

作者： Li, Zhong-Yuan;Guo, Hai-Ting;Tan, Jie;Li, Zhong-Yuan;Geng, Zhao-Yu;Li, Zhong-Yuan;Zhu, Xing-Quan;Zhu, Xing-Quan

作者机构：

关键词： Toxoplasma gondii; IMP1; Virulence; CRISPR/Cas9; Intracellular proliferation

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.011； 五年影响因子：2.132 ）

ISSN： 0014-4894

年卷期： 2020 年 211 卷

页码：

收录情况： SCI

摘要： The intracellular protozoan Toxoplasma gondii infects approximately one-third of the world's population as well as various animals, causing toxoplasmosis. However, there remains a need to define the functions of newly identified genes of T. gondii. In the present study, a novel molecule, immune mapped protein 1 of T. gondii (TgIMP1), was devitalized by CRISPR/Cas9 system to investigate the phenotypic changes of the parasite. We found that the virulence of Delta TgIMP1 knockout strain was reduced in comparison with wild-type GT1 tachyzoites, showing a statistically decreased plaque in HFF cells and a significantly prolonged survival period of mice (P < 0.05). Moreover, the data of phenotype analyses in vitro showed a different level of the intracellular proliferation and the subsequent egress between Delta TgIMP1 and wild-type GT1 strain (P < 0.05); while no statistically significant difference was detected during the process of attachment or invasion. These results suggested that TgIMP1 is closely associated with the intracellular proliferation of this parasite.

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