Genetic organization of genes encoding phenol hydroxylase, benzoate 1,2-dioxygenase alpha subunit and its regulatory proteins in Acinetobacter calcoaceticus PHEA-2
文献类型: 外文期刊
第一作者: Xu, YQ
作者: Xu, YQ;Chen, M;Zhang, W;Lin, M
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关键词: Complete nucleotide-sequence;Pseudomonas sp strain-cf600;Transcriptional activator;Functional-analysis;Positive regulator;Catabolic pathway;Degradation;Putida;Catechol;Expression
期刊名称:CURRENT MICROBIOLOGY ( 影响因子:2.188; 五年影响因子:2.197 )
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收录情况: SCI
摘要: Acinetobacter calcoaceticus PHEA-2 is a phenol-degrading bacterium isolated from the wastewater from an oil refinery. A 10-kb XhoI fragment consisting of nine complete Open Reading Frames (ORFs) and one partial ORF was screened from a lambda library of PHEA-2 by Southern hybridization. The sequence analyses revealed that ORF2-ORF7, designated mphKLMNOP, are homologous to dmpKLMNOP of Pseudomonas sp. CF600 and mopKLMNOP of Acinetobacter calcoaceticus NCIB8250, sharing 38%-72% and 58.5%-93.5% respectively. The products encoded by dMP and mop genes convert phenol to catechol. The mph-operon and downstream ORFs; ORF9 and ORF10, sharing high identities to benM and benA, which encode ben-operon regulatory protein and benzoate 1,2-dioxygenase alpha subunit respectively, are separated by ORF8, whose function is unknown. The organization of the mph and ben operons is different from that described previously.
分类号: Q93
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