Isolation and expression analysis of LEA genes in peanut (Arachis hypogaea L.)

文献类型： 外文期刊

第一作者： Su, Lei

作者： Su, Lei;Bi, Yu-Pin;Wang, Xing-Jun;Su, Lei;Zhao, Chuan-Zhi;Bi, Yu-Pin;Wan, Shu-Bo;Xia, Han;Wang, Xing-Jun;Su, Lei;Zhao, Chuan-Zhi;Bi, Yu-Pin;Wan, Shu-Bo;Xia, Han;Wang, Xing-Jun;Su, Lei;Zhao, Chuan-Zhi;Bi, Yu-Pin;Wan, Shu-Bo;Xia, Han;Wang, Xing-Jun

作者机构：

关键词： Arachis hypogaea L.; cDNA library; ESTs; late embryogenesis abundant protein

期刊名称：JOURNAL OF BIOSCIENCES （ 影响因子：1.826； 五年影响因子：2.107 ）

ISSN： 0250-5991

年卷期： 2011 年 36 卷 2 期

页码：

收录情况： SCI

摘要： Late embryogenesis abundant (LEA) protein family is a large protein family that includes proteins accumulated at late stages of seed development or in vegetative tissues in response to drought, salinity, cold stress and exogenous application of abscisic acid. In order to isolate peanut genes, an expressed sequence tag (EST) sequencing project was carried out using a peanut seed cDNA library. From 6258 ESTs, 19 LEA-encoding genes were identified and could be classified into eight distinct groups. Expression of these genes in seeds at different developmental stages and in various peanut tissues was analysed by semi-quantitative RT-PCR. The results showed that expression levels of LEA genes were generally high in seeds. Some LEA protein genes were expressed at a high level in non-seed tissues such as root, stem, leaf, flower and gynophore. These results provided valuable information for the functional and regulatory studies on peanut LEA genes.

分类号： Q

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