Molecular cloning and expression analysis of FvMYB1 from Fraxinus velutina Torr.

文献类型： 外文期刊

第一作者： Li, Tian

作者： Li, Tian;Bi, Yu-Ping;Li, Tian;Peng, Zhen-Ying;Bi, Yu-Ping;Fan, Zhong-Xue;Li, Tian;Peng, Zhen-Ying;Bi, Yu-Ping;Fan, Zhong-Xue;Li, Tian

作者机构：

关键词： Arizona ash; expression analysis; molecular cloning; salt stress; V-myb avian myeloblastosis viral oncogene homolog

期刊名称：TURKISH JOURNAL OF AGRICULTURE AND FORESTRY （ 影响因子：2.585； 五年影响因子：1.974 ）

ISSN： 1300-011X

年卷期： 2013 年 37 卷 5 期

页码：

收录情况： SCI

摘要： V-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors play important roles in the processes of plant growth, development, and stress responses. In this study, a full-length cDNA sequence of a MYB gene, designated FvMYB1, was isolated from Arizona ash (Fraxinus velutina Torr.) for the first time. Sequence analysis showed that the deduced amino acid sequence of FvMYB1 encoded novel MYB proteins with single DNA binding domains. Based on the R3 domain amino acid sequence, the FvMYB1 was closely related to some proteins whose functions were known. The expression pattern of FvMYB1 in different organs of Arizona ash was analyzed by semiquantitative RT-PCR and real-time PCR (qRT-PCR). Results showed that this gene was widely distributed in all the tested organs and the expression level of FvMYB1 was the highest in stems and the lowest in roots. The gene expression level decreased dramatically in roots under salt treatment (300 mM, 24 h), while no obvious change was observed in stems. Subcellular localization indicated that FvMYB1 was localized in the nucleus. This study will lead to further research in resistance to abiotic stress in Arizona ash.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序