Rapid and accurate detection of Salmonella enterica serovar Enteritidis using a one-step LAMP-CRISPR/Cas12b method

文献类型： 外文期刊

第一作者： Gong, Jiansen

作者： Gong, Jiansen;Li, Tingting;Dou, Xinhong;Zhang, Di;Xu, Jingxiao;Zhang, Zhipeng;Wang, Chengming

作者机构：

关键词： Salmonella Enteritidis; CRISPR/Cas12b; LAMP; PCR

期刊名称：FOOD CONTROL （ 影响因子：6.0； 五年影响因子：5.8 ）

ISSN： 0956-7135

年卷期： 2024 年 164 卷

页码：

收录情况： SCI

摘要： Salmonella enterica serovar Enteritidis ( S . Enteritidis) poses a significant threat as a prevalent foodborne pathogen globally. Timely diagnostic methods are essential for effective management of S . Enteritidis infections. Herein, we establish a one-step LAMP-CRISPR/Cas12b method for a rapid, accurate, and convenient detection of S . Enteritidis. The entire reaction requires only 45 min without the need for specialized equipment. The platform exhibited 100% specificity with no cross -reactivity observed among 19 other Salmonella serovars and 9 nonSalmonella bacterial pathogens. Our method achieved a limit of detection of 30 copies per reaction. Compared with the traditional culture methods in practical analysis. S . Enteritidis positivity rates identified by one-step LAMP-CRISPR/Cas12b platform were 2.88% (6/208) including 3.14% (4/127) in chicken, 4.44% (2/45) in duck, and 0.0% (0/36) in pork, and a 100% concordance was observed between the traditional culture method and one-step LAMP-CRISPR/Cas12b platform. In summary, our findings underscore the simplicity and accuracy of the one-step LAMP-CRISPR/Cas12b platform for S . Enteritidis detection, with potential applications in resource -limited settings.

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