Selection and use of microsatellite markers for individual identification and meat traceability of six swine breeds in the Chinese market

文献类型： 外文期刊

第一作者： Zhao, Jie

作者： Zhao, Jie;Li, Tingting;Zhu, Chao;Jiang, Xiaoling;Zhao, Yan;Xu, Zhenzhen;Yang, Shuming;Chen, Ailiang;Zhao, Jie;Li, Tingting;Zhu, Chao;Jiang, Xiaoling;Zhao, Yan;Xu, Zhenzhen;Yang, Shuming;Chen, Ailiang

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关键词： Microsatellite markers; animal identification; pork traceability; Chinese market

期刊名称：FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL （ 影响因子：2.023； 五年影响因子：2.24 ）

ISSN： 1082-0132

年卷期： 2018 年 24 卷 4 期

页码：

收录情况： SCI

摘要： Meat traceability based on molecular markers is exerting a great influence on food safety and will enhance its key role in the future. This study aimed to investigate and verify the polymorphism of 23 microsatellite markers and select the most suitable markers for individual identification and meat traceability of six swine breeds in the Chinese market. The mean polymorphism information content value of these 23 loci was 0.7851, and each locus exhibited high polymorphism in the pooled population. There were 10 loci showing good polymorphism in each breed, namely, Sw632, S0155, Sw2406, Sw830, Sw2525, Sw72, Sw2448, Sw911, Sw122 and CGA. When six highly polymorphic loci were combined, the match probability value for two random individual genotypes among the pig breeds (Beijing Black, Sanyuan and Taihu) was lower than 1.151 E-06. An increasing number of loci indicated a gradually decreasing match probability value and therefore enhanced traceability accuracy. The validation results of tracing 18 blood and corresponding meat samples based on five highly polymorphic loci (Sw2525, S0005, Sw0107, Sw911 and Sw857) were successful, with 100% conformation probability, which provided a foundation for establishing a traceability system for pork in the Chinese market.

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