Combinatorial multispectral, thermodynamics, docking and site-directed mutagenesis reveal the cognitive characteristics of honey bee chemosensory protein to plant semiochemical

文献类型： 外文期刊

第一作者： Tan, Jing

作者： Tan, Jing;Song, Xinmi;Fu, Xiaobin;Wu, Fan;Li, Hongliang;Hu, Fuliang;Wu, Fan

作者机构：

关键词： Honey bee; Chemosensory protein; Multispectral analysis; Thermodynamics; Molecular docking; Site-directed mutagenesis

期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY （ 影响因子：4.098； 五年影响因子：3.464 ）

ISSN： 1386-1425

年卷期： 2018 年 201 卷

页码：

收录情况： SCI

摘要： In the chemoreceptive system of insects, there are always some soluble binding proteins, such as some antennal-specific chemosensory proteins (CSPs), which are abundantly distributed in the chemosensory sensillar lymph. The antenna]-specific CSPs usually have strong capability to bind diverse semiochemicals, while the detailed interaction between CSPs and the semiochemicals remain unclear. Here, by means of the combinatorial multispectral, thermodynamics, docking and site-directed mutagenesis, we detailedly interpreted a binding interaction between a plant semiochemical beta-ionone and antennal-specific CSP1 from the worker honey bee. Thermodynamic parameters (Delta H< 0,Delta S> 0) indicate that the interaction is mainly driven by hydrophobic forces and electrostatic interactions. Docking prediction results showed that there are two key amino acids, Phe44 and GIn63, may be involved in the interacting process of CSP1 to beta-ionone. In order to confirm the two key amino acids, site-directed mutagenesis were performed and the binding constant (K-A) for two CSP1 mutant proteins was reduced by 60.82% and 46.80% compared to wild-type CSP1. The thermodynamic analysis of mutant proteins furtherly verified that Phe44 maintained an electrostatic interaction and GIn63 contributes hydrophobic and electrostatic forces. Our investigation initially elucidates the physicochemical mechanism of the interaction between antennal-special CSPs in insects including bees to plant semiochemicals, as well as the development of twice thermodynamic analysis (wild type and mutant proteins) combined with multispectral and site-directed mutagenesis methods. (C) 2018 Elsevier B.V. All rights reserved.

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