Isolation of two novel promoters from ramie (Boehmeria nivea L. Gaudich) and its functional characterization in Arabidopsis thaliana

文献类型： 外文期刊

第一作者： Guo, Pingan

作者： Guo, Pingan;Zheng, Yancheng;Chen, Jie;Wang, Bo;Liu, Lijun;Peng, Dingxiang;Feng, Enying

作者机构：

关键词： Ramie; Promoter; Cis-acting element; Abiotic stress

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期： 2019 年 136 卷 3 期

页码：

收录情况： SCI

摘要： The development of tissue-specific or inducible promoters is important for plant genetic engineering. In this study, we isolated two novel promoters (named BnGDS(pro) and BnSTMpro) from ramie and analyzed their functions in Arabidopsis. The results show that BnGDS(pro) and its 5-truncated fragments drive GUS dominantly expressed in shoot meristem while BnSTMpro and its 5-truncated fragments have apical meristem specificity in Arabidopsis seedlings and nodule specificity in the flowers and siliques. The -1233 to -914 region and -425 to 0 region of BnSTMpro are responsible for apical root specificity and root-knot specificity, respectively. In particular, CuSO4 treatment reveals that both BnGDS(pro) and BnSTMpro are copper-inducible promoters. Our findings suggest that BnGDS(pro) and BnSTMpro and their truncated fragments have potential applications in plant genetic transformation.

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