文献类型: 外文期刊
第一作者: Gong, Wankui
作者: Gong, Wankui;Zhou, Yun;Wang, Rui;Wei, Xiaoli;Zhang, Lei;Dai, Yan;Zhu, Zhen
作者机构:
关键词: Transgenic rice; T-DNA integration; Microhomology; Filler DNA; Translocation; Cut; paste mechanism
期刊名称:JOURNAL OF PLANT PHYSIOLOGY ( 影响因子:3.549; 五年影响因子:4.164 )
ISSN: 0176-1617
年卷期: 2021 年 266 卷
页码:
收录情况: SCI
摘要: Agrobacterium-mediated plant transformation has been widely used for introducing transgene(s) into a plant genome and plant breeding. However, our understanding of T-DNA integration into rice genome remains limited relative to that in the model dicot Arabidopsis. To better elucidate the T-DNA integration into the rice genome, we investigated extensively the T-DNA ends and their flanking rice genomic sequences from two transgenic rice plants carrying Cowpea Trypsin Inhibitor (CpTI)-derived gene Signal-CpTI-KDEL (SCK) and Bacillus thuringiensis (BT) gene, respectively, by TAIL-PCR method. Analysis of the junction sequences between the T-DNA ends and rice genome DNA indicated that there were three joining patterns of microhomology, filler DNA sequences, and exact joining, and both the T-DNA ends tend to adopt identical manner to join the rice genome. After T-DNA integration, there were several variations of rice genomic sequences, including small deletions at the integration sites, superfluous DNA inserted between T-DNA and genome, and translocation of genomic DNA in the flanking regions. The translocation block could be from a noncontiguous region in the same chromosome or different chromosomes at the integration sites, and the originating position of the translocated block resulted in comparable deletion based on a cut/paste mechanism rather than a replication mechanism. Our study may lead to a better understand of T-DNA integration mechanism and facilitate functional genomic studies and further crop improvement.
分类号:
- 相关文献
作者其他论文 更多>>
-
Characterization of rhizosphere bacterial communities in oilseed rape cultivars with different susceptibility to Plasmodiophora brassicae infection
作者:Deng, Yue;Huang, Xiaoqing;Yang, Xiaoxiang;Yu, Yaoyin;Zhang, Zhongmei;Hu, Zijin;Zhou, Xiquan;Liu, Yong;Zhang, Lei;Deng, Yue;Wu, Wenxian;Huang, Xiaoqing;Yang, Xiaoxiang;Yu, Yaoyin;Zhang, Zhongmei;Liu, Yong;Zhang, Lei;Zhou, Kang;Zhou, Kang
关键词:rhizosphere microbiome;
Plasmodiophora brassicae ; susceptible cultivar; resistant cultivar; microbial metabolism; nitrogen cycle -
Biodegradable film drip fertigation is more conducive to reducing the diversity and abundance of antibiotic resistance genes than plastic film drip fertigation
作者:Li, Yingjie;Yuan, Yuhan;Li, Cuilan;Zhang, Jinjing;Liu, Hang;Wang, Meng;Li, Qian;Zhang, Lei;Sun, Bo;Qin, Yubo
关键词:Antibiotic resistance genes; Biodegradable film drip fertigation; Bacterial diversity; HT-PCR
-
Para-coumaric acid and cinnamic acid enhance resistance of Agaricus bisporus s mushrooms to Brown blotch disease caused by Pseudomonas tolaasii
作者:Shi, Zixuan;Song, Rui;Zhang, Lei;Jiang, Hanyue;Jiao, Lu;Yuan, Shuai;Meng, Demei;Zheng, Yanyan;Chen, Lin
关键词:Bacterial disease; Edible mushroom; Induced resistance; Phenolic acid; Storage
-
Jatrophane diterpenoids from Jatropha curcas with multidrug resistance reversal activity
作者:He, Yi-Lin;Li, Guo-Li;Yang, Hong-Ying;Zhang, Lei;Gong, Zheng;He, Yi-Lin;Zhang, Lan-Jun;Yang, Hong-Ying;Gao, Kun;Zhang, Lan-Jun
关键词:Jatropha curcas L.; Euphorbiaceae; Jatrophane diterpenoids; Multidrug resistance reversal activities
-
Black soybean peptide mediates the AMPK/SIRT1/NF-κB signaling pathway to alleviate Alzheimer's-related neuroinflammation in lead-exposed HT22 cells
作者:Yang, Huijie;Zhao, Peijun;Ullah, Saif;Ma, Yan;Zhao, Guangshan;Cheng, Yongxia;Li, Qian;Li, Tiange;Qiao, Mingwu;Song, Lianjun;Zhang, Lei;Huang, Xianqing;Li, Ning;Wang, Fangyu;Galaverna, Gianni
关键词:Black soybean peptide; Lead (Pb); Alzheimer's disease; Neuroinflammation; AMPK/SIRT1/NF-kappa B pathway
-
Diversity and distribution of Mdace mutations involved in propoxur resistance in the house fly (Musca domestica L.) in China
作者:You, Chunmei;Ji, Xiangyun;You, Chunmei;Zhang, Lulu;Song, Jiajia;Zhen, Congai;Zhang, Lei;Gao, Xiwu
关键词:Musca domestica; propoxur; acetylcholinesterase; Mdace; mutation
-
Risk Assessment of Cyclaniliprole Resistance in Peach-Potato Aphid Myzus persicae: Laboratory Selection, Inheritance, and Cross-Resistance Patterns
作者:Liang, Pingzhuo;Zhang, Zihao;Zhen, Congai;Zhang, Lei;Li, Dapeng;Cheng, Shenhang;Li, Ren
关键词:cross-resistance; cyclaniliprole; inheritance;
Myzus persicae ; reproduction fitness; resistance selection; risk assessment