Evaluation of five regions as DNA barcodes for identification of Lepista species (Tricholomataceae, Basidiomycota) from China

文献类型： 外文期刊

第一作者： Wang, Siyu

作者： Wang, Siyu;Li, JiaJia;Li, Wei;Yu, Xiaodan;Guo, Hongbo;Wang, Qin

作者机构：

关键词： Agaricales; Intra-specific diversity; DNA barcoding; Inter-specific diversity; Species delineation

期刊名称：PEERJ （ 影响因子：3.061； 五年影响因子：3.537 ）

ISSN： 2167-8359

年卷期： 2019 年 7 卷

页码：

收录情况： SCI

摘要： Background: Distinguishing among species in the genus Lepista is difficult because of their similar morphologies. Methods: To identify a suitable DNA barcode for identification of Lepista species, we assessed the following five regions: internal transcribed spacer (ITS), the intergenic spacer (IGS), nuclear ribosomal RNA subunit, mitochondria) small subunit rDNA, and tefl. A total of 134 sequences from 34 samples belong to eight Lepista species were analyzed. The utility of each region as a DNA barcode was assessed based on the success rates of its PCR amplification and sequencing, and on its intra- and inter-specific variations. Results: The results indicated that the ITS region could distinguish all species tested. We therefore propose that the ITS region can be used as a DNA barcode for the genus Lepista. In addition, a phylogenetic tree based on the ITS region showed that the tested eight Lepista species, including two unrecognized species, formed eight separate and well-supported clades.

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