Clone of Chinese Jinan red-cross yellow cattle and evaluation of reproductive characteristics of cloned calf

文献类型： 外文期刊

第一作者： Dai, YP

作者： Dai, YP;Lian, ZX;Zhu, HB;Gong, GC;Wang, LL;Wang, HP;Zhao, ZH;Zhu, QH;Fei, J;Li, N

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关键词： Chinese Jinan red-cross yellow cattle;somatic nuclear transfer;livestock conservation;embryo activation

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： Somatic cell done technology is a viable approach to preserving endangered livestock and wildlife genetic resources. la the present research, somatic cell nuclear transfer (SCNT) was performed using granulose cells from the critical endangered Chinese red-cross yellow cattle as donor cells. A total of 211 ocytes were manipulated and 166 v) of them were successfully enucleated. 112 (67.4 percent) embryos were reconstructed, 94 (83 percent) of them cleaved, and 48 (43 percent) of them developed to blastocyst stage. SCNT blastocysts were transferred to 6 Holstein recipients, and 2 (33 percent) of them were found to be pregnant. One of them maintained to term and delivered a calf, whereas another aborted. Effect of different fusion buffer (mannitol vs. Zimmerman fusion buffer) and different. activation methods (calcium ionophore+6-DMAP vs. cydoheximide +CB) on fusion rate and development of SCNT embryos were investigated. The results indicated that: (i) on condition of two DC pulses of 2.5 kV/cm for 18 fis each, fusion rates were higher in mannitol solution than in Zimmerman fusion buffer (71 percent vs. 61 percent, respectively, < 0.05), but the blastocysts rates did not differ between two treatments (36 percent vs. 39 percent, p>0.05 ); (ii) There was no significant difference in development rates to the blastocyst stage for SCNT embryos activated by calcium ionophore+6-DMAP or by cycloheximideCB (42 percent vs. 46 percent, respectively, p>0.05). Microsatellite DNA analysis examining 28 loci confirmed that the cloned calf was genetically identical to the donor Jinan red-cross yellow cattle and different from the recipient females. Growth and reproductive performance of cloned cow were evaluated, and there were no difference i cross-red n it between cloned and normal control Jinan yellow cattle. Furthermore, the cloned yellow cow has delivered a healthy yellow calf.

分类号： N1

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