Phosphomannose-isomerase (pmi) gene as a selectable marker for rice transformation via Agrobacterium
文献类型: 外文期刊
第一作者: He, ZQ
作者: He, ZQ;Fu, YP;Si, HM;Hu, GC;Zhang, SH;Yu, YH;Sun, ZX
作者机构: China Natl Rice Res Inst, Minist Agr, Key Lab Rice Biol, Hangzhou 310006, Peoples R China;China Natl Rice Res Inst, Minist Agr, Key Lab Rice Biol, Hangzhou 310006, Peoples R China; Zhejiang Univ, Coll Life Sci, Hangzhou 310029, Peoples R China; Guizhou Univ, Coll Biotechnol, Guiyang 550025, Peoples R China; Chinese Agr Univ, Beijing, Peoples R China
关键词: Transformation;Oryza sativa;Phosphomannose-isomerase;Positive;Transgenic plant-cells;Zea-mays l;Positive selection;Sugar-beet;Xylose isomerase;Maize plants;Mannose;Mechanism;Tumefaciens;Inhibition
期刊名称:PLANT SCIENCE ( 2020影响因子:4.729; 五年影响因子:5.132 )
收录情况: SCI
摘要: A new selectable marker system was adapted for use in Agrobacterium-mediated transformation of rice (Oryza sativa L.). This selection system utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. Only transformed cells were capable of utilizing mannose as a carbon source. Transgenic rice plants regenerated from selected transformed immature embryo-derived calli on media containing various concentrations of mannose. The highest transformation frequency of 6.0% was obtained when a combination of 25 g/l mannose and 5 g/l sucrose was used. Molecular and genetic analyses showed that the plants contained the pmi gene and the gene was transmitted to the progeny in Mendelian fashion. The results indicated that the mannose selection system, which was devoid of the disadvantages of antibiotic or herbicide selection, could be used for rice Agrobacterium-mediated immature embryo transformation
分类号: Q94
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