Phosphomannose-isomerase (pmi) gene as a selectable marker for rice transformation via Agrobacterium

文献类型: 外文期刊

第一作者: He, ZQ

作者: He, ZQ;Fu, YP;Si, HM;Hu, GC;Zhang, SH;Yu, YH;Sun, ZX

作者机构: China Natl Rice Res Inst, Minist Agr, Key Lab Rice Biol, Hangzhou 310006, Peoples R China;China Natl Rice Res Inst, Minist Agr, Key Lab Rice Biol, Hangzhou 310006, Peoples R China; Zhejiang Univ, Coll Life Sci, Hangzhou 310029, Peoples R China; Guizhou Univ, Coll Biotechnol, Guiyang 550025, Peoples R China; Chinese Agr Univ, Beijing, Peoples R China

关键词: Transformation;Oryza sativa;Phosphomannose-isomerase;Positive;Transgenic plant-cells;Zea-mays l;Positive selection;Sugar-beet;Xylose isomerase;Maize plants;Mannose;Mechanism;Tumefaciens;Inhibition

期刊名称:PLANT SCIENCE ( 2020影响因子:4.729; 五年影响因子:5.132 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A new selectable marker system was adapted for use in Agrobacterium-mediated transformation of rice (Oryza sativa L.). This selection system utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. Only transformed cells were capable of utilizing mannose as a carbon source. Transgenic rice plants regenerated from selected transformed immature embryo-derived calli on media containing various concentrations of mannose. The highest transformation frequency of 6.0% was obtained when a combination of 25 g/l mannose and 5 g/l sucrose was used. Molecular and genetic analyses showed that the plants contained the pmi gene and the gene was transmitted to the progeny in Mendelian fashion. The results indicated that the mannose selection system, which was devoid of the disadvantages of antibiotic or herbicide selection, could be used for rice Agrobacterium-mediated immature embryo transformation

分类号: Q94

  • 相关文献

[1]High-efficiency Agrobacterium-mediated transformation of Lotus corniculatus L. using phosphomannose isomerase positive selection. Guo, Qianqian,Ma, Jiangtao,Yuan, Bei,Wu, Yanmin,Guo, Qianqian,Ma, Jiangtao,Zhou, Meiliang.

[2]Mannose selection system used for cucumber transformation. He, Zhengquan,Duan, ZhenZhen,Liang, Wei,Chen, Faju,Yao, Wei,Liang, Hongwei,Yue, Chaoyin,Sun, Zongxiu,Chen, Fan,Dai, Jianwu. 2006

[3]ELEVATED ENDOGENOUS ISOPENTENYL ADENINE CONTENT IS CORRELATED WITH AN EXTREMELY SHOOTY RICE PHENOTYPE. ZHU, YX,QIN, RZ,SHAN, XY,CHEN, ZL.

[4]Inhibitory effect and mechanism of mesenchymal stem cells on liver cancer cells. Hou, Lingling,Wang, Xiaoyu,Zhou, Yaqiong,Ma, Haibin,Wang, Ziling,He, Jinsheng,Hu, Honggang,Guan, Weijun,Ma, Yuehui. 2014

[5]Inhibition of isoflavone biosynthesis enhanced T-DNA delivery in soybean by improving plant-Agrobacterium tumefaciens interaction. Zhang, Yan-Min,Zhang, Hong-Mei,Liu, Zi-Hui,Guo, Xiu-Lin,Li, Hui-Cong,Li, Guo-Liang,Jiang, Chun-Zhi,Zhang, Meng-Chen.

[6]Engineered Bacillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests. Wang, Guangjun,Zhang, Jie,Song, Fuping,Wu, Jun,Feng, Shuliang,Huang, Dafang.

[7]Water stress induces in pear leaves the rise of betaine level that is associated with drought tolerance in pear. Gao, XP,Yan, JY,Liu, EK,Shen, YY,Lu, YF,Zhang, DP.

[8]An unusual posttranscriptional processing in two Betaine aldehyde dehydrogenase loci of cereal crops directed by short, direct repeats in response to stress conditions. Zheng, Wenjing,Lu, Bao-Rong,Ren, Guangjun,Huang, Weizao,Wang, Songhu,Liu, Junli,Tang, Zizhi,Luo, Di,Wang, Yuguo,Liu, Yongsheng.

[9]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[10]Independent Losses of Function in a Polyphenol Oxidase in Rice: Differentiation in Grain Discoloration between Subspecies and the Role of Positive Selection under Domestication. Tang, Tian,Wu, Chung-I,Shi, Suhua,Yu, Yanchun,Wang, Yonghong,Li, Jiayang,Yu, Yanchun,Wang, Yonghong,Li, Jiayang,Qian, Qian,Yan, Meixian,Zeng, Dali,Han, Bin,Han, Bin,Wu, Chung-I,Wu, Chung-I.

[11]Genome-wide identification and evolutionary analysis of positively selected miRNA genes in domesticated rice. Liu, Qingpo,Wang, Hong,Hu, Haichao,Liu, Qingpo,Wang, Hong,Hu, Haichao,Zhang, Hengmu.

[12]Rapid diversification of five Oryza AA genomes associated with rice adaptation. Zhang, Qun-Jie,Zhu, Ting,Xia, En-Hua,Shi, Chao,Liu, Yun-Long,Zhang, Yun,Liu, Yuan,Jiang, Wen-Kai,Zhao, You-Jie,Mao, Shu-Yan,Zhang, Li-Ping,Huang, Hui,Jiao, Jun-Ying,Xu, Ping-Zhen,Yao, Qiu-Yang,Yang, Li-Li,Gao, Ju,Gao, Li-Zhi,Zhang, Qun-Jie,Zhu, Ting,Xia, En-Hua,Shi, Chao,Liu, Yuan,Yao, Qiu-Yang,Zeng, Fan-Chun,Gao, Ju,Tao, Da-Yun,Wang, Yue-Ju,Bennetzen, Jeffrey L..

[13]Evidence for positive selection in Ser/Thr protein kinases (STKs) genes of Trichodesmium erythraeum. Liang, Chengwei,Zhang, Xiaowen. 2009

[14]De novo assembly and characterization of the Hucho taimen transcriptome. Tong, Guang-Xiang,Xu, Wei,Zhang, Yong-Quan,Zhang, Qing-Yu,Yin, Jia-Sheng,Kuang, You-Yi. 2018

[15]Gene duplication and an accelerated evolutionary rate in 11S globulin genes are associated with higher protein synthesis in dicots as compared to monocots. Li, Chun,Li, Meng,Zhang, Yuan-Ming,Li, Chun,Dunwell, Jim M.. 2012

[16]Population size may shape the accumulation of functional mutations following domestication. Chen, Jianhai,Ni, Pan,Li, Xinyun,Zhao, Shuhong,Chen, Jianhai,Ni, Pan,Li, Xinyun,Zhao, Shuhong,Han, Jianlin,Han, Jianlin,Jakovlic, Ivan,Zhang, Chengjun. 2018

[17]The impact of positive selection and mutation in prevalence of representative O/Mya-98 foot and mouth disease strains during 2009-2010. Kuang, Wendong,Bai, Xingwen,Qi, Guocai,Hao, Xiaofang,Li, Pinghua,Lu, Zengjun,Bao, Huifang,Sun, Pu,Wu, Lei,Liu, Zaixin. 2012

[18]Selective modes determine evolutionary rates, gene compactness and expression patterns in Brassica. Liu, Jing,Du, Jianchang,Zhang, Jiefu,Du, Jianchang,Liu, Shengyi,Du, Jianchang. 2017

[19]A genome-wide scan for signatures of selection in Chinese indigenous and commercial pig breeds. Yang, Songbai,Li, Kui,Tang, Zhonglin,Yang, Songbai,Li, Xiuling,Fan, Bin,Yang, Songbai,Li, Xiuling,Fan, Bin. 2014

[20]Evolution and molecular epidemiology of foot-and-mouth disease virus in China. Bai XingWen,Li PingHua,Bao HuiFang,Liu ZaiXin,Li Dong,Lu ZengJun,Cao YiMei,Shang YouJun,Shao JunJun,Chang HuiYun,Luo JianXun,Liu XiangTao. 2011

作者其他论文 更多>>