Isolation and characterization of polycyclic aromatic hydrocarbons-degrading Sphingomonas sp strain ZL5

文献类型： 外文期刊

第一作者： Liu, YS

作者： Liu, YS;Zhang, J;Zhang, ZZ

作者机构：

关键词： Biodegradation;Catechol 2;3-dioxygenase;Degradative plasmid;Pseudomonas-putida;Catabolic plasmid;Phenanthrene;Naphthalene;Metabolism;Bacteria;Gene;Biodegradation;Identification;Dioxygenase

期刊名称：BIODEGRADATION （ 影响因子：3.909； 五年影响因子：3.578 ）

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收录情况： SCI

摘要： A bacterial strain ZL5, capable of growing on phenanthrene as a sole carbon and energy source but not naphthalene, was isolated by selective enrichment from crude-oil-contaminated soil of Liaohe Oil Field in China. The isolate was identified as a Sphingomonas sp. strain on the basis of 16S ribosomal DNA analysis. Strain ZL5 grown on phenanthrene exhibited catechol 2,3-dioxygenase (C23O) activity but no catechol 1,2-dioxygenase, gentisate 1,2-dioxygenase, protocatechuate 3,4-dioxygenase and protocatechuate 4,5-dioxygenase activities. This suggests that the mode of cleavage of phenanthrene by strain ZL5 could be meta via the intermediate catechol, which is different from the protocatechuate way of other two bacteria, Alcaligenes faecelis AFK2 and Nocardioides sp. strain KP7, also capable of growing on phenanthrene but not naphthalene. A resident plasmid (approximately 60 kb in size), designated as pZL, was detected from strain ZL5. Curing the plasmid with mitomycin C and transferring the plasmid to E. coli revealed that pZL was responsible for polycyclic aromatic hydrocarbons degradation. The C23O gene located on plasmid pZL was cloned and overexpressed in E. coli JM109(DE3). The ring-fission activity of the purified C23O from the recombinant E. coli on dihydroxylated aromatics was in order of catechol > 4-methylcatechol > 3-methylcatechol > 4-chlorocatechol much greater than 3,4-dihydroxyphenanthrene > 3-chlorocatechol.

分类号： Q5

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