Isolation and characterization of polycyclic aromatic hydrocarbons-degrading Sphingomonas sp strain ZL5

文献类型： 外文期刊

第一作者： Liu, YS

作者： Liu, YS;Zhang, J;Zhang, ZZ

作者机构：

关键词： Biodegradation;Catechol 2;3-dioxygenase;Degradative plasmid;Pseudomonas-putida;Catabolic plasmid;Phenanthrene;Naphthalene;Metabolism;Bacteria;Gene;Biodegradation;Identification;Dioxygenase

期刊名称：BIODEGRADATION （ 影响因子：3.909； 五年影响因子：3.578 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A bacterial strain ZL5, capable of growing on phenanthrene as a sole carbon and energy source but not naphthalene, was isolated by selective enrichment from crude-oil-contaminated soil of Liaohe Oil Field in China. The isolate was identified as a Sphingomonas sp. strain on the basis of 16S ribosomal DNA analysis. Strain ZL5 grown on phenanthrene exhibited catechol 2,3-dioxygenase (C23O) activity but no catechol 1,2-dioxygenase, gentisate 1,2-dioxygenase, protocatechuate 3,4-dioxygenase and protocatechuate 4,5-dioxygenase activities. This suggests that the mode of cleavage of phenanthrene by strain ZL5 could be meta via the intermediate catechol, which is different from the protocatechuate way of other two bacteria, Alcaligenes faecelis AFK2 and Nocardioides sp. strain KP7, also capable of growing on phenanthrene but not naphthalene. A resident plasmid (approximately 60 kb in size), designated as pZL, was detected from strain ZL5. Curing the plasmid with mitomycin C and transferring the plasmid to E. coli revealed that pZL was responsible for polycyclic aromatic hydrocarbons degradation. The C23O gene located on plasmid pZL was cloned and overexpressed in E. coli JM109(DE3). The ring-fission activity of the purified C23O from the recombinant E. coli on dihydroxylated aromatics was in order of catechol > 4-methylcatechol > 3-methylcatechol > 4-chlorocatechol much greater than 3,4-dihydroxyphenanthrene > 3-chlorocatechol.

分类号： Q5

  • 相关文献

[1]Biodegradation of phenanthrene by Rhizobium petrolearium SL-1. Huang, X.,Zhang, R.,Ma, X.,Zhang, X.,Huang, X.,Zhang, R.,Shi, J.,Cui, C.,Yin, H..

[2]Phytodegradation of organophosphorus compounds by transgenic plants expressing a bacterial organophosphorus hydrolase. Wang, Xiaoxue,Wu, Ningfeng,Guo, Jun,Chu, Xiaoyu,Tian, Jian,Fan, Yunliu,Yao, Bin.

[3]Biodegradation of pyrene and benzo[a]pyrene in the liquid matrix and soil by a newly identified Raoultella planticola strain. Ping, Lifeng,Yuan, Xiaoli,Ping, Lifeng,Guo, Qian,Chen, Xiaoyang,Zhang, Chunrong,Zhao, Hua. 2017

[4]Biological degradation of triclocarban and triclosan in a soil under aerobic and anaerobic conditions and comparison with environmental fate modelling. Ying, Guang-Guo,Ying, Guang-Guo,Yu, Xiang-Yang,Kookana, Rai S.,Yu, Xiang-Yang.

[5]Degradation of four fungicides in tropical soils from Hainan, China. Han, Bing Jun,Peng, Li Xu,Chen, Li Xia.

[6]Association of different endosymbionts with the whitefly species Bemisia tabaci and Trialeurodes vaporariorum (Sternorrhyncha : Aleyrodidae). Tan, ZJ,Xie, BY,Xiao, QM,Yang, YH,Wan, FH,Huang, SW.

[7]Transgenic ice nucleation-active Enterobacter cloacae reduces cold hardiness of corn borer and cotton bollworm larvae. Tang, CR,Sun, FZ,Zhang, XJ,Zhao, TC,Qi, JY.

[8]Biodegradation of nicosulfuron by a Talaromyces flavus LZM1. Song, Jinlong,Wu, Wei,Wang, Haisheng,Shi, Yanhua,Yan, Yanchun,Gu, Jingang,Ruan, Zhiyong,Zhai, Yi.

[9]Molecular cloning, purification and biochemical characterization of a novel pyrethroid-hydrolyzing carboxylesterase gene from Ochrobactrum anthropi YZ-1. Zhai, Yi,Li, Kang,Song, Jinlong,Shi, Yanhua,Yan, Yanchun.

[10]Isolation and characterization of Bacillus amyloliquefaciens ZDS-1: Exploring the degradation of Zearalenone by Bacillus spp.. Xu, Jianhong,Wang, Hongjie,Zhu, Ziwei,Ji, Fang,Yin, Xianchao,Shi, Jianrong,Zhu, Ziwei,Hong, Qing.

[11]Biodegradation of naproxen by freshwater algae Cymbella sp and Scenedesmus quadricauda and the comparative toxicity. Yang, Bo,Yang, Mengting,Li, Juying,Ding, Tengda,Lin, Kunde,Li, Wenying,Gan, Jay,Yang, Bo,Li, Juying.

[12]Functional identification of gene cluster for the aniline metabolic pathway mediated by transposable element. Liang, QF,Chen, M,Xu, YQ,Zhang, W,Ping, SZ,Lu, W,Song, XL,Wang, WW,Geng, LZ,Masahiro, T,Lin, M.

[13]Isolation and characterization of Methanoculleus receptaculi sp nov from Shengli oil field, China. Cheng, Lei,Qiu, Tian-Lei,Li, Xia,Deng, Yu,Yin, Xiao-Bo,Zhang, Hui,Qiu, Tian-Lei,Wang, Wei-Dong.

[14]Biodegradation of insecticide carbofuran by Paracoccus sp YM3. Yan, Yan C.,Peng, Xiang,Zhang, Jing S.,Li, Ying Y.,Li, Wen,Peng, Xiang,Zhang, Jing S.,Li, Ying Y.,Li, Wen,Xu, Gang M.,Xu, Gang M..

[15]Characterization of a novel nitrilase, BGC4, from Paraburkholderia graminis showing wide-spectrum substrate specificity, a potential versatile biocatalyst for the degradation of nitriles. Fan, Haiyang,Chen, Lifeng,Wang, Hualei,Liu, Qinghai,Ren, Yuhong,Wei, Dongzhi,Sun, Huihui.

[16]Biodegrading of Pyrene by a Newly Isolated Pseudomonas putida PL2. Ping, Lifeng,Zhang, Chunrong,Zhu, Yahong,Wu, Min,Hu, Xiuqing,Li, Zhen,Zhao, Hua.

[17]Characterization of a novel oxyfluorfen-degrading bacterial strain Chryseobacterium aquifrigidense and its biochemical degradation pathway. Xu, Jun,Dong, Fengshou,Liu, Xingang,Wu, Xiaohu,Zheng, Yongquan,Wu, Yanbing.

[18]Esterase activity inspired selection and characterization of zearalenone degrading bacteria Bacillus pumilus ES-21. Wang, Gang,Yu, Mingzheng,Dong, Fei,Shi, Jianrong,Xu, Jianhong.

[19]Biological decolorization of malachite green by Deinococcus radiodurans R1. Lv, Guo-Ying,Cheng, Jian-Hui,Zhang, Zuo-Fa,Fan, Lei-Fa,Cheng, Jian-Hui,Chen, Xiao-Yang.

[20]Biodegradation of TNT using Bacillus mycoides immobilized in PVA-sodium alginate-kaolin. Lin, Hongyan,Chen, Zuliang,Lin, Hongyan,Chen, Zuliang,Megharaj, Mallavarapu,Naidu, Ravendra,Chen, Zuliang,Megharaj, Mallavarapu,Naidu, Ravendra.

作者其他论文 更多>>

微信小程序