Chromosome-encoded gene cluster for the metabolic pathway that converts aniline to TCA-cycle intermediates in Delftia tsuruhatensis AD9

文献类型： 外文期刊

第一作者： Liang, O

作者： Liang, O;Takeo, M;Chen, M;Zhang, W;Xul, YQ;Lin, M

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期刊名称：MICROBIOLOGY-SGM （ 影响因子：2.777； 五年影响因子：2.871 ）

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收录情况： SCI

摘要： Delftia tsuruhatensis AD9 was isolated as an aniline-degrading bacterium from the soil surrounding a textile dyeing plant.The gene cluster involved in aniline degradation was cloned from the total DNA of strain AD9 into Escherichia coli JM109.After shotgun cloning,two recombinant E.coli strains showing aniline oxidation activity or catechol meta-cleavage activity were obtained by simple plate assays.These strains contained 9.3 kb and 15.4 kb DNA fragments,respectively.Sequence analysis of the total 24.7 kb region revealed that this region contains a gene cluster (consisting of at least 1 7 genes,named tad QTA1A2BRD1C1D2C2EFGIJKL) responsible for the complete metabolism of aniline to TCA-cycle intermediates.In the gene cluster,the first five genes (tadQTA1A2B) and the subsequent gene (tadR) were predicted to encode a multi-component aniline dioxygenase and a LysR-type regulator,respectively,while the others (tadD1C1D2C2EFGIJKL) were expected to encode mefa-cleavage pathway enzymes for catechol degradation.In addition,it was found that the gene cluster is surrounded by two 1S1071 sequences,indicating that it has a class I transposon-like structure.PFGE and Southern hybridization analyses confirmed that the fad gene cluster is encoded on the chromosome of strain AD9 in a single copy.These results suggest that,in strain AD9,aniline is degraded via catechol through a mefa-cleavage pathway by the chromosome-encoded fad gene cluster.The fad gene cluster showed significant similarity in nucleotide sequence and genetic organization to the plasmid-encoded aniline degradation gene cluster of Pseudomonas putida UCC22.

分类号： Q93

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