Robust RNAi-Based Resistance to Mixed Infection of Three Viruses in Soybean Plants Expressing Separate Short Hairpins from a Single Transgene

文献类型： 外文期刊

第一作者： Zhang, Xiuchun

作者： Zhang, Xiuchun;Dorrance, Anne E.;Qu, Feng;Zhang, Xiuchun;Sato, Shirley;Clemente, Thomas E.;Sato, Shirley;Clemente, Thomas E.;Ye, Xiaohong;Morris, T. Jack;Ye, Xiaohong;Morris, T. Jack

作者机构：

关键词： double-stranded RNA;transgenes;35S promoter

期刊名称：PHYTOPATHOLOGY （ 影响因子：4.025； 五年影响因子：4.394 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Transgenic plants expressing double-stranded RNA (dsRNA) of virus origin have been previously shown to confer resistance to virus infections through the highly conserved RNA-targeting process termed RNA silencing or RNA interference (RNAi). In this study we applied this strategy to soybean plants and achieved robust resistance to multiple viruses with a single dsRNA-expressing transgene. Unlike previous reports that relied on the expression of one long inverted repeat (IR) combining sequences of several viruses, our improved strategy utilized a transgene designed to express several shorter IRs. Each of these short IRs contains highly conserved sequences of one virus, forming dsRNA of less than ISO bp. These short dsRNA stems were interspersed with single-stranded sequences to prevent homologous recombination during the transgene assembly process. Three such short IRs with sequences of unrelated soybean-infecting viruses (Alfalfa mosaic virus, Bean pod mottle virus, and Soybean mosaic virus) were assembled into a single transgene under control of the 35S promoter and terminator of Cauliflower mosaic virus. Three independent transgenic lines were obtained and all of them exhibited strong systemic resistance to the simultaneous infection of the three viruses. These results demonstrate the effectiveness of this very straight forward strategy for engineering RNAi-based virus resistance in a major crop plant. More importantly, our strategy of construct assembly makes it easy to incorporate additional short IRs in the transgene, thus expanding the spectrum of virus resistance. Finally, this strategy

分类号： S432.1

  • 相关文献

[1]Overexpression of Arabidopsis CBF1 gene in transgenic tobacco alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance. Yang, Jia-Sen,Meng, Jing-Jing,Bi, Yu-Ping,Xu, Ping-Li,Guo, Feng,He, Qi-Wei,Li, Xin-Guo,Yang, Jia-Sen,Meng, Jing-Jing,Bi, Yu-Ping,Xu, Ping-Li,Guo, Feng,Wan, Shu-Bo,Li, Xin-Guo,Wang, Ran,Wang, Ran.

[2]Advances in the biosafety research of non-target DNA in transgenic plants. Ma, RC,Peng, YF,Guan, HS. 2001

[3]Use of a novel metal indicator to judge loop-mediated isothermal amplification for detecting the 35S promoter. Wang, Xiaofu,Chen, Xiaoyun,Peng, Cheng,Xu, Xiaoli,Wei, Wei,Xu, Junfeng,Wang, Xiaofu,Chen, Xiaoyun,Peng, Cheng,Xu, Xiaoli,Wei, Wei,Xu, Junfeng,Fu, Zhenfang,Li, Feiwu.

[4]RNA interference of a putative S-adenosyl-L-homocysteine hydrolase gene affects larval performance in Leptinotarsa decemlineata (Say). Zhou, Li-Tao,Jia, Shuang,Wan, Pin-Jun,Kong, Ye,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun. 2013

[5]RNA interference of E75 nuclear receptor gene suppresses transmission of rice stripe virus in Laodelphax striatellus. Fang, Ying,Lee, Seok Hee,Kim, Jong Hoon,Park, Dong Hwan,Park, Min Gu,Woo, Ra Mi,Lee, Bo Ram,Kim, Woo Jin,Je, Yeon Ho,Choi, Jae Young,Je, Yeon Ho,Li, Shuo. 2017

[6]RNAi-mediated plant protection against aphids. Yu, Xiu-Dao,Sun, Yong-Wei,Ma, You-Zhi,Xia, Lan-Qin,Yu, Xiu-Dao,Liu, Zong-Cai,Huang, Si-Liang,Chen, Zhi-Qin,Duan, Peng-Fei.

[7]Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae). Tian, Huixia,Yu, Shijiang,Yue, Jiansu,Ran, Chun,Yang, Juan,Ding, Lili,Liu, Haoqiang,Li, Hongjun,Cong, Lin,Ran, Chun,Liu, Bin,Chen, Fei.

[8]Using double-stranded RNA to explore the role of heat shock protein genes in heat tolerance in Bemisia tabaci (Gennadius). Lue, Zhi-Chuang,Wan, Fang-Hao,Wan, Fang-Hao.

[9]Hunchback IS REQUIRED FOR ABDOMINAL IDENTITY SUPPRESSION AND GERMBAND GROWTH IN THE PARTHENOGENETIC EMBRYOGENESIS OF THE PEA APHID, Acyrthosiphon pisum. Liu, Changyan,Zeng, Fanrong.

[10]Response of the vacuolar ATPase subunit E to RNA interference and four chemical pesticides in Leptinotarsa decemlineata (Say). Fu, Kai-Yun,Lu, Feng-gong,Liu, Xin-ping,Li, Guo-Qing,Guo, Wen-Chao.

作者其他论文 更多>>

微信小程序