Karyotype reconstruction modulates the sensitivity of barley genome to radiation-induced DNA and chromosomal damage

文献类型： 外文期刊

第一作者： Stoilov, Lubomir

作者： Stoilov, Lubomir;Georgieva, Mariyana;Manova, Vasilissa;Gecheff, Kostadin;Liu, Luxiang

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期刊名称：MUTAGENESIS （ 影响因子：3.0； 五年影响因子：3.679 ）

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收录情况： SCI

摘要： The potential of cytologically reconstructed barley line D-2946 to cope with the major lesions that hamper genome integrity, namely DNA single- and double-strand breaks was investigated. Strand breaks induced by ??-rays and Li ions were assessed by neutral and alkaline comet assay. Repair capacity after bleomycin treatment was evaluated by agarose gel electrophoresis under neutral and alkaline conditions. Frequencies of radiation-induced chromosome aberrations were also determined. Results indicate that radiation-mediated constitutive rearrangement of the chromosome complement has led to a substantial modulation of the sensitivity of barley genome towards DNA strand breaks, produced by ionising radiation, Li ion implantation and bleomycin in an agent-specific manner, as well as of the clastogenic response to ??-rays. Based on these findings, reconstructed barley karyotype D-2946 can be considered a candidate radio-sensitive line with reduced ability to maintain genome integrity with respect to both DNA and chromosomal damage. ? The Author 2012. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved.

分类号： R394

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