Purification and characterization of a novel acid phosphatase from the split gill mushroom Schizophyllum commune

文献类型： 外文期刊

第一作者： Zhang, Guo-Qing

作者： Zhang, Guo-Qing;Chen, Qing-Jun;Sun, Jian;Wang, He-Xiang;Sun, Jian;Wang, He-Xiang;Han, Chun-Hua

作者机构：

关键词： Acid phosphatase;Chromatography;Kinetics;N-terminal amino acid sequence;Schizophyllum commune

期刊名称：JOURNAL OF BASIC MICROBIOLOGY （ 影响因子：2.281； 五年影响因子：2.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A monomeric acid phosphatase (ACP) with a molecular mass of 72.5kDa was purified from fresh fruiting bodies of cultured Schizophyllum commune mushroom. The isolation procedure entailed ion exchange chromatography on DEAE-cellulose, CM-cellulose, and Q-sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. It demonstrated a unique N-terminal amino acid sequence of NAPWAQIDEV, which exhibited 60% amino acid identity to that of S. commune hypothetical histidine ACP based on its genome sequence, but less than 30% amino acid identity to that of other fungal ACPs previously reported. The ACP exhibited an optimum temperature at 50°C, an optimum pH at pH 4.6, and was considerably stable at a pH range of 4.0 to 9.0, and a temperature range of 20-40°C. The Km of the purified enzyme for ρ-nitrophenyl phosphate (ρNPP) was 0.248mM and the Vmax was 9.093×10-3μM/min. ACP activity was strongly inhibited by Al3+ and Fe3+, but enhanced by Co2+, Mg2+, and Ca2+ at a concentration of 0.5mM.

分类号： R37

  • 相关文献

[1]Schizolysin, a hemolysin from the split gill mushroom Schizophyllum commune. Ng, Tzi Bun,Han, Chun-Hua,Han, Chun-Hua,Zhang, Guo-Qing,Wang, He-Xiang.

[2]A novel ribonuclease with potent HIV-1 reverse transcriptase inhibitory activity from cultured mushroom Schizophyllum commune. Zhang, Guo-Qing,Wang, He-Xiang,Zhang, Guo-Qing,Wang, He-Xiang,Zhao, Yong-Chang,Zhang, Guo-Qing,Ng, Tzi-Bun.

[3]Potassium release rates from ustisols and their application. Lu, XN,Zhang, MH,Xu, JM.

[4]Two novel antimicrobial peptides purified from the symbiotic bacteria Xenorhabdus budapestensis NMC-10. Xiao, Yao,Meng, Fanlu,Qiu, Dewen,Yang, Xiufen.

[5]Primary purification of two antifungal proteins from leaves of the fig (Ficus carica L.). Pan, Ying-hong,Zhao, Ming,Ma, Yan,Yuan, Wen-xia,Yan, Wei. 2011

[6]Separation of Recombinant Geranylgeranyl Diphosphate Synthase of Deinococcus radiodurans from Expressed Strain Cell Homogenate by Immobilized Metal Affinity Chromatography on a Characterized Monolithic Cryogel Column. Shen Shaochuan,Wang Liangyan,Li Mingfeng,Liu Chengzhi,Tian Bing,Hua Yuejin,Shen Shaochuan,Yun Junxian,Sun Zongtao. 2013

[7]Separation of flavonol glycosides from Flaveria bidentis (L.) Kuntze by high-speed counter-current chromatography. Xie, Qianqian,Wei, Yun,Zhang, Guoliang.

[8]Removal of acidic interferences in multi-pesticides residue analysis of fruits using modified magnetic nanoparticles prior to determination via ultra-HPLC-MS/MS. Qi, Peipei,Wang, Zhiwei,Yang, Guiling,Xu, Hao,Wang, Xiangyun,Zhang, Hu,Wang, Qiang,Wang, Xinquan,Shang, Chunqing,Qi, Peipei,Wang, Zhiwei,Yang, Guiling,Wang, Xinquan,Xu, Hao,Wang, Xiangyun,Zhang, Hu,Wang, Qiang.

[9]HIGH LEVELS OF ERGONOVINE AND LYSERGIC ACID AMIDE IN TOXIC ACHNATHERUM INEBRIANS ACCOMPANY INFECTION BY AN ACREMONIUM-LIKE ENDOPHYTIC FUNGUS. Miles, CO,Lane, GA,diMenna, ME,Garthwaite, I,Piper, EL,Ball, OJP,Latch, GCM,Allen, JM,Hunt, MB,Bush, LP,Min, FK,Fletcher, I,Harris, PS.

[10]Isolation and characterization of an antifungal protein from Bacillus licheniformis HS10. Wang, Zhixin,Zheng, Li,Yang, Xiaona,Liu, Hongxia,Guo, Jianhua,Wang, Zhixin,Zheng, Li,Yang, Xiaona,Liu, Hongxia,Guo, Jianhua,Wang, Zhixin,Zheng, Li,Yang, Xiaona,Liu, Hongxia,Guo, Jianhua,Wang, Yunpeng,Zheng, Li,Zheng, Li.

[11]Effects of different enzymatic hydrolysis methods on the bioactivity of peptidoglycan in Litopenaeus vannamei. Song Xiaoling,Zhang Yue,Wei Song,Huang Jie,Zhang Yue. 2013

[12]Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene (Ha-acp1) from Heterodera avenae. Huang Wen-kun,Long Hai-bo,Peng Huan,He Wen-ting,Peng De-liang. 2014

[13]Characterization of acid phosphatase from the tick Haemaphysalis longicornis. Zhang, Ping,Tian, Zhancheng,Liu, Guangyuan,Xie, Junren,Luo, Jin,Zhang, Liyan,Shen, Hui. 2011

[14]Effect of ginger extract and vinegar on ATP metabolites, IMP-related enzyme activity, reducing sugars and phosphorylated sugars in silver carp during postslaughter storage. Shi, Ce,Cui, Jianyun,Qin, Na,Luo, Yongkang,Lu, Han,Wang, Hang,Shi, Ce,Luo, Yongkang.

[15]A novel antimicrobial protein isolated from potato (Solanum tuberosum) shares homology with an acid phosphatase. Yuan, FH,Gao, Y,Liang, CG,Xu, J,Zhang, CL,He, LY.

[16]Effects of mycorrhizal fungi on phytate-phosphorus utilization in trifoliate orange (Poncirus trifoliata L. Raf) seedlings. Shu, Bo,Wang, Peng,Xia, Ren-Xue,Shu, Bo,Shu, Bo.

[17]Spatial distribution and expression of intracellular and extracellular acid phosphatases of cluster roots at different developmental stages in white lupin. Tang, Hongliang,Li, Xiaoqing,Zu, Chao,Zhang, Fusuo,Shen, Jianbo,Tang, Hongliang,Zu, Chao.

[18]Characterisation of the phytase gene in trifoliate orange (Poncirus trifoliata (L.) Raf.) seedlings. Shu, Bo,Wang, Peng,Xia, Ren-Xue,Shu, Bo,Shu, Bo.

[19]Mechanochemistry in Thermomechanical Processing of Foods: Kinetic Aspects. Zhao, Xuewei,Wang, Zhangcun,Zhang, Peiqi,Wei, Yimin,Zhang, Bo,Chen, Fengliang. 2011

[20]Radiation-induced reduction of chromium(VI) in aqueous solution by gamma-irradiation in a laboratory-scale. Yuan, SJ,Zheng, Z,Mu, YY,Yu, X,Zhao, YF. 2006

作者其他论文 更多>>

微信小程序