Recovery of Native Protein from Potato Root Water by Expanded Bed Adsorption with Amberlite XAD7HP

文献类型： 外文期刊

第一作者： Zeng, Fan-Kui

作者： Zeng, Fan-Kui;Ma, Peng-Jun;Liu, Gang;Liu, Hong;Ma, Peng-Jun

作者机构：

关键词： potato root water;potato protein;protease inhibitors;expanded bed adsorption;Amberlite XAD7HP

期刊名称：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING （ 影响因子：2.836； 五年影响因子：2.281 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Potato root water (PRW) contains ～1.5% protein. In this study, expanded bed adsorption (EBA) chromatography with Amberlite XAD7HP resin adsorbent was used to isolate native protein from crude PRW. The optimal pH and ionic strength for potato protein binding onto Amberlite XAD7HP were 5.0 and 20 mmol/L. The EBA-refined proteins were dried by vacuum freeze drying and spray drying at varying outlet temperatures. Results indicated that low temperature spray drying was the most cost effective method with respect to retaining protease inhibitor activities. The dried protein concentrates appeared bright yellow or dark reddish brown, with a total glycoalkaloid content of ～ 170 μg/g. The protease inhibitor activity was ～ 400 mg/g and 11～ 12 mg/g for trypsin inhibition and chymotrypsin inhibition, respectively. The results presented here suggest that EBA using Amberlite XAD7HP as the adsorbent is a feasible strategy for the direct adsorption of native protein from crude PRW.

分类号： Q

  • 相关文献

[1]Calorimetric, rheological, and structural properties of potato protein and potato starch composites and gels. Zhang, Duqin,Mu, Taihua,Sun, Hongnan,Zhang, Duqin,Mu, Taihua,Sun, Hongnan.

[2]Processing of delta-endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1 in Heliothis armigera midgut juice and the effects of protease inhibitors. Shao, ZZ,Cui, YL,Liu, XL,Yi, HQ,Ji, JH,Yu, ZN. 1998

[3]Transgenic Strategies for Enhancement of Nematode Resistance in Plants. Ali, Muhammad A.,Abbas, Amjad,Ali, Muhammad A.,Joyia, Faiz A.,Azeem, Farrukh,Li, Hongjie,Dababat, Abdelfattah A.. 2017

作者其他论文 更多>>

微信小程序