Functional Characterization of aroA from Rhizobium leguminosarum with Significant Glyphosate Tolerance in Transgenic Arabidopsis

文献类型： 外文期刊

第一作者： Han, Jing

作者： Han, Jing;Tian, Yong-Sheng;Xu, Jing;Wang, Li-Juan;Wang, Bo;Peng, Ri-He;Yao, Quan-Hong

作者机构：

关键词： 5-Enolpyruvylshikimate-3-phosphate synthase;Rhizobium leguminosarum;glyphosate tolerant;transgenic Arabidopsis

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

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年卷期：

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收录情况： SCI

摘要： Glyphosate is the active component of the top-selling herbicide, the phytotoxicity of which is due to its inhibition of the shikimic acid pathway. 5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) is a key enzyme in the shikimic acid pathway. Glyphosate tolerance in plants can be achieved by the expression of a glyphosate-insensitive aroA gene (EPSPS). In this study, we used a PCR-based two-step DNA synthesis method to synthesize a new aroA gene (aroA_(R. leguminosarum)) from Rhizobium leguminosarum. In vitro glyphosate sensitivity assays showed that aroA_(R. leguminosarum) is glyphosate tolerant. The new gene was then expressed in E. coli and key kinetic values of the purified enzyme were determined. Furthermore, we transformed the aroA gene into Arabidopsis thaliana by the floral dip method. Transgenic Arabidopsis with the aroA_(R. leguminosarum) gene was obtained to prove its potential use in developing glyphosate-resistant crops.

分类号： Q93

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