A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of bifenthrin in a chemical soil barrier

文献类型： 外文期刊

第一作者： Hua, Xiude

作者： Hua, Xiude;Liu, Xiaofeng;Yin, Wei;Shi, Haiyan;Liu, Fengquan;Wang, Minghua;Hua, Xiude;Yin, Wei;Shi, Haiyan;Wang, Minghua;Xia, Yazhong;Zhou, Qiujun;Lu, Yiwen;Li, Wei;Liu, Fengquan

作者机构：

关键词： Bifenthrin;Enzyme-linked immunosorbent assay;Chemical soil barrier;Monoclonal antibody

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：7.963； 五年影响因子：7.842 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A sensitive monoclonal antibody-based enzyme monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) was developed to detect bifenthrin in a chemical soil barrier for termite control. LBc ((2-methyl [1,1-biphenyl]-3-methoxy) carbonyl propionic acid) was conjugated to bovine serum albumin (BSA) for producing monoclonal antibody. After optimization, the IC50 and limit of detection (LOD, IC10) were 0.05 mg L-1 and 0.004 mg L-1, respectively. The sensitivity was improved 40-fold compared to polyclonal antibody-based ELISA reported earlier. No cross-reactivity was measured for the other analogues such as cyhalothrin, cyhalothric acid, cypermethrin, deltamethrin, fenpropathrin, fenvalerate, ethofenprox and tetramethrin except 2-methyl-3-biphenylmethyanol. Spiked recoveries were between 83.5% and 104.7% for the detection of bifenthrin in loess, red soil and black soil. All the relative standard deviations (RSDs) were less than or equal to 15.0%. Moreover, the ELISA for authentic samples showed reliability and high correlation with gas chromatography. The developed ELISA is an alternative tool for simple, sensitive and accurate monitoring of bifenthrin in chemical soil barriers. (C) 2014 Elsevier B.V. All rights reserved.

分类号： X1

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