Inhibition of migration and induction of apoptosis in LoVo human colon cancer cells by polysaccharides from Ganoderma lucidum

文献类型： 外文期刊

第一作者： Liang, Zeng-Enni

作者： Liang, Zeng-Enni;Wang, Ren-Cai;Hu, Qiu-Long;Xiong, Xing-Yao;Yi, You-Jin;Guo, Yu-Tong

作者机构：

关键词： Ganoderma lucidum polysaccharides;human colon cancer;apoptosis;death receptor pathway

期刊名称：MOLECULAR MEDICINE REPORTS （ 影响因子：2.952； 五年影响因子：2.754 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Ganoderma lucidum polysaccharides (GLPs), which were purified from the medicinal herb G. lucidum followed by ethanol precipitation, protein depletion using the Sevage assay, purification using DEAE-cellulose (DE-52), dialysis and the use of ultrafiltration membranes, are used as an ingredient in traditional anticancer treatments in China. The aim of the current study was to evaluate the anticancer effects and investigate the underlying molecular mechanisms of GLPs on LoVo human colon cancer cells. The results demonstrated that the GLP-mediated anticancer effect in LoVo cells was characterized by cytotoxicity, migration inhibition, enhanced DNA fragmentation, morphological alterations and increased lactate dehydrogenase release. Furthermore, the activation of caspases-3, -8 and -9 was involved in GLP-stimulated apoptosis. Additionally, treatment with GLPs promoted the expression of Fas and caspase-3 proteins, whilst reducing the expression of cleaved poly(ADP-ribose) polymerase. These data indicate that GLPs demonstrate potential antitumor activity in human colon cancer cells, predominantly through the inhibition of migration and induction of apoptosis. Furthermore, activation of the Fas/caspase-dependent apoptosis pathway is involved in the cytotoxicity of GLPs.

分类号： R3

  • 相关文献

[1]Anti-inflammatory and hepatoprotective effects of Ganoderma lucidum polysaccharides on carbon tetrachloride-induced hepatocyte damage in common carp (Cyprinus carpio L.). Liu, Ying-Juan,Jia, Rui,Shen, Yu-Jin,Zhao, Cai-Yuan,Yin, Guo-Jun,Du, Jin-Liang,Cao, Li-Ping,Xu, Pao,Yin, Guo-Jun,Du, Jin-Liang,Cao, Li-Ping,Xu, Pao,Yin, Guo-Jun. 2015

[2]Effect of heat stress on production and in-vitro antioxidant activity of polysaccharides in Ganoderma lucidum. Tan, Xiaoyan,Li, Hengchang,Hu, Jing,Ning, Huijuan,Qin, Zifang,Zhang, Xiuqing,Sun, Junshe,Pei, Haisheng,Xu, Zhangyang,Sun, Tian. 2018

[3]Development of liposomal Ganoderma lucidum polysaccharide: Formulation optimization and evaluation of its immunological activity. Liu, Zhenguang,Huang, Yee,Bo, Ruonan,Gao, Zhenzhen,Yu, Yun,Hu, Yuanliang,Liu, Jiaguo,Wu, Yi,Wang, Deyun,Ma, Xia,Deng, Bihua.

[4]The Caspase Pathway of Linoelaidic Acid (9t, 12t-C18:2)-Induced Apoptosis in Human Umbilical Vein Endothelial Cells. Bin, Qiu,Rao, Huan,Hu, Jiang-Ning,Liu, Rong,Fan, Ya-Wei,Li, Jing,Deng, Ze-Yuan,Zhong, Xianfeng,Bin, Qiu,Du, Fang-Ling. 2013

[5]Detection of DNA ladder during cotyledon senescence in cotton. Q.E. XIE,I.D. LIU,S.X. YU,R.F. WANG,Z.X. FAN,Y.G. WANG,F.F. SHEN.

[6]Effects of species and cellular activity of oviductal epithelial cells on their dialogue with co-cultured mouse embryos. Tan, Xiu-Wen,Ma, Suo-Feng,Yu, Jian-Ning,Zhang, Xia,Lan, Guo-Cheng,Liu, Xin-Yong,Han, Zheng-Bin,Tan, Jing-He. 2007

[7]Oxidative Stress-Mediated Cytotoxicity of Cadmium in Chicken Splenic Lymphocytes. Li, Jin-Long,Tang, Zhao-Xin,Li, Jin-Long,Li, Shu,Xu, Shi-Wen,Li, Hui-Xin,Wang, Xiao-Long. 2010

[8]Heat stress inhibits proliferation, promotes growth, and induces apoptosis in cultured Lantang swine skeletal muscle satellite cells. Gao, Chun-qi,Zhao, Yin-ling,Sui, Wei-guo,Yan, Hui-chao,Wang, Xiu-qi,Li, Hai-chang. 2015

[9]Facile fabrication of graphene oxide loaded with silver nanoparticles as antifungal materials. Cui, Jianghu,Yang, Yunhua,Zheng, Mingtao,Liu, Yingliang,Xiao, Yong,Lei, Bingfu,Chen, Wei. 2014

[10]Mitophagy promotes replication of oncolytic Newcastle disease virus by blocking intrinsic apoptosis in lung cancer cells. Meng, Gang,Xia, Mao,Wang, Diancheng,Chen, Aiping,Wang, Hongwei,Yu, Decai,Wei, Jiwu,Yu, Decai,Wei, Jiwu,Wang, Yongshan. 2014

[11]Induction of apoptosis in the SW620 colon carcinoma cell line by triterpene-enriched extracts from Ganoderma lucidum through activation of caspase-3. Ji, Zhe,Tang, Qingju,Hao, Ruixa,Zhang, Jinsong,Ji, Zhe,Pan, Yingje. 2011

[12]Molecular characterization of SjBIRP, another apoptosis inhibitor, from Schistosoma japonicum. Dao, Jinwei,Zhu, Lihui,Luo, Rong,Hu, Chao,Wang, Yuqing,Li, Hao,Lu, Ke,Liu, Jinming,Lin, Jiaojiao,Cheng, Guofeng,Dao, Jinwei,Cheng, Guofeng. 2014

[13]Inhibition of p38 mitogen-activated protein kinase phosphorylation decreases H2O2-induced apoptosis in human lens epithelial cells. Bai, Jie,Zheng, Yi,Dong, Li,Liu, Ping,Cai, Xuehui,Wang, Gang. 2015

[14]Sperm pretreatment with glutathione improves IVF embryos development through increasing the viability and antioxidative capacity of sex-sorted and unsorted bull semen. Hu Ting-xi,Wang Zong-li,Hu Ting-xi,Zhu Hua-bin,Sun Wei-jun,Hao Hai-sheng,Zhao Xue-ming,Du Wei-hua. 2016

[15]Resveratrol induces apoptosis and inhibits adipogenesis by stimulating the SIRT1-AMPK alpha-FOXO1 signalling pathway in bovine intramuscular adipocytes. Liu, Xiaomu,Zhao, Hongbo,Jin, Qing,You, Wei,Cheng, Haijian,Liu, Yifan,Song, Enliang,Liu, Guifen,Tan, Xiuwen,Zhang, Xianglun,Wan, Fachun,Liu, Xiaomu,Zhao, Hongbo,Jin, Qing,You, Wei,Cheng, Haijian,Liu, Yifan,Song, Enliang,Liu, Guifen,Tan, Xiuwen,Zhang, Xianglun,Wan, Fachun. 2018

[16]BMP15 Prevents Cumulus Cell Apoptosis Through CCL2 and FBN1 in Porcine Ovaries. Zhai, Bo,Liu, Huiyu,Dai, Lisheng,Gao, Yan,Li, Changhong,Zhang, Lianjiang,Yu, Xianfeng,Zhang, Jiabao,Li, Xu. 2013

[17]Protective Effects of Quercetin on Cadmium-induced Cytotoxicity in Primary Cultures of Rat Proximal Tubular Cells. Wang Lin,Liu Gang,Wang Zhen Yong,Lin Shu Qian,He Yuan Long. 2013

[18]Effect of SIRT1 on Apoptosis of Bovine Preadipocytes. Liu Xiao-Mu,Song En-Liang,Liu Gui-Fen,Tan Xiu-Wen,Liu Zhen-Shan,Wan Fa-Chun,Liu Xiao-Mu,Song En-Liang,Liu Gui-Fen,Tan Xiu-Wen,Liu Zhen-Shan,Wan Fa-Chun,Wu Gong-Ying. 2010

[19]AKT signalling and mitochondrial pathways are involved in mushroom polysaccharide-induced apoptosis and G(1) or S phase arrest in human hepatoma cells. OuYang, Fengju,Wang, Guibin,Zhang, Yuanyuan,Zhao, Min,Guo, Wei,Xiang, Wenhua. 2013

[20]RIP1 is a central signaling protein in regulation of TNF-alpha/TRAIL mediated apoptosis and necroptosis during Newcastle disease virus infection. Liao, Ying,Mao, Xiang,Wang, Huang,Li, Yanrong,Sun, Yingjie,Meng, Chun,Tan, Lei,Song, Cuiping,Qiu, Xusheng,Ding, Chan,Ding, Chan,Wang, Hua-xia,Fang, Hongjie. 2017

作者其他论文 更多>>

微信小程序