Molecular detection and genetic characterization of Toxoplasma gondii infection in sika deer (Cervus nippon) in China

文献类型： 外文期刊

第一作者： Cong, Wei

作者： Cong, Wei;Qin, Si-Yuan;Zhu, Xing-Quan;Cong, Wei;Qian, Ai-Dong;Qin, Si-Yuan;Meng, Qing-Feng;Zou, Feng-Cai;Zhu, Xing-Quan

作者机构：

关键词： Toxoplasma gondii;Sika deer;Genetic characterization;China

期刊名称：INFECTION GENETICS AND EVOLUTION （ 影响因子：3.342； 五年影响因子：3.188 ）

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收录情况： SCI

摘要： The objective of the present study was to investigate the prevalence and genetic characterization of Toxoplasma gondii infection in sika deer in China. During August 2014 to November 2014, a total of 450 tissue samples coming from 150 sika deer were collected to detect the T. gondii B1 gene using a nested PCR, and the positive samples were genotyped at 11 genetic markers (SAG1, 5'- and 3'-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and Apico) using multilocus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. Seventeen of 150 sika deer (11.33%) were tested positive by nested PCR. Six DNA samples from the 17 positive samples were completely typed, in which 4 samples from lung tissues, and 2 from muscular tissues, were identified as ToxoDB Genotype #9 (http://toxodb.org/toxo/). The results of the present study revealed the existence of T. gondii infection in sika deer in China, which provided the information of T. gondii genetic diversity in this host species. This study also indicated that ToxoDB Genotype #9 has a wide distribution in sika deer that could be potential reservoirs for T. gondii transmission, which may pose a threat to human health. (C) 2016 Elsevier B.V. All rights reserved.

分类号： R1

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