Differentiation of chicken umbilical cord mesenchymal stem cells into beta-like pancreatic islet cells

文献类型: 外文期刊

第一作者: Bai, Chunyu

作者: Bai, Chunyu;Gao, Yuhua;Li, Qian;Feng, Yuan;Yu, Yanze;Meng, Gentong;Zhang, Minghai;Bai, Chunyu;Gao, Yuhua;Li, Qian;Guan, Weijun

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关键词: chicken;islet cells;mesenchymal stem cells;umbilical cord

期刊名称:ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY ( 影响因子:5.678; 五年影响因子:5.246 )

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收录情况: SCI

摘要: In this study, we explored the possibility of using in vitro differentiation to create functional beta-like islet cells from chicken umbilical cord mesenchymal stem cells (UCMSCs). Passaged UCMSCs were induced to differentiate into pancreatic beta-like islet cells. Differentiated cells were observed through dithizone staining, and Pdx1 and insulin expressed in differentiated cells were detected with immunofluorescence. Insulin and C-peptide production from differentiated cells were analyzed using ELISA and western blotting. Differentiated cells were found to not only express Pdx1, insulin, and C-peptide, but also to display a glucose-responsive secretion of these hormones.

分类号: R31

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