A high throughput method for rapid screening of chitosanase-producing fungal strain under acidic conditions

文献类型： 外文期刊

第一作者： Chen, Gui-Guang

作者： Chen, Gui-Guang;Liang, Zhi-Qun;Zeng, Wei;Chen, Gui-Guang;Liang, Zhi-Qun;Zeng, Wei;Chen, Gui-Guang;Liang, Zhi-Qun;Zeng, Wei;Cao, Mu-Ming;Chen, Guo-Pin;Xie, Shu-Yu;Li, Wei

作者机构：

关键词： High-throughput method;Chitooligosaccharides;Chitosanase;Aspergillus fumigatus

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

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收录情况： SCI

摘要： A novel high-throughput method was established for rapid screening of a large numbers of Aspergillus fumigatus ( A. fumigatus) mutants with high chitosanase production under acidic culture condition by exploiting the fact that iodine can be used as the indicator to stain chitosan but is ineffective for chitooligosaccharides. The mutant population was generated by irradiating A. fumigatus CICC 2434 with Co-60-gamma rays. Mutants were cultured on acidic plates containing colloidal chitosan and preliminary screened according to diameter of haloes formed around colonies. Then, chitosanase production of the isolates were verified by dinitrosalicylic acid assay. Lastly, molecular masses on enzymolysis products of isolated mutants were rapidly compared by aniline blue plate assay. Using this method, the mutant strain Co-8 was selected, which had chitosanase activity of 24.87 U/mL ( increased by 369.2 % as compared to that of its parental strain). Taking together, the method is easy, efficient and particularly suited to rapid screen acidophilic fungal strains with high chitosanase-production.

分类号： Q93`Q-33

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