Genetic variability among Hymenolepis nana isolates from different geographical regions in China revealed by sequence analysis of three mitochondrial genes

文献类型： 外文期刊

第一作者： Cheng, Tian

作者： Cheng, Tian;Lin, Rui-Qing;Cheng, Tian;Gao, De-Zhen;Zhu, Wei-Ning;Zhu, Xing-Quan;Liu, Guo-Hua;Cheng, Tian;Lin, Rui-Qing;Gao, De-Zhen;Fang, Su-Fang;Chen, Ning;Liu, Guo-Hua

作者机构：

关键词： Hymenolepis nana;mitochondrial DNA (mtDNA);sequence variation;phylogenetic analysis

期刊名称：MITOCHONDRIAL DNA PART A （ 影响因子：1.514； 五年影响因子：0.707 ）

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收录情况： SCI

摘要： Hymenolepis nana is a common tapeworm that parasitizes in the small intestine of rodent animals and humans. The present study examined the sequence diversity of three mitochondrial (mt) genes namely NADH dehydrogenase subunits 5 (nadS), small subunitribosomal RNA {rrnS), and ATPase subunit 6 (atp6) of H. nana from mice in different geographical regions of China. A part of the nadS (pnadS), complete rrnS and atp6 genes were amplified separately from individual H. nana isolates using polymerase chainreaction (PCR) and then sequenced. The sequences of pnadS, rrnS, and atp6 were 710bp, 704-711 bp, and 516bp in length, respectively. The A + T contents of the sequences were 70.1-73.5% (pnadS), 70.1-71.7% (rrnS), and 76.6-77.9% (<3fp6). Sequence variation within H. nana was 0-1.4% for atp6, 0-1.7% for rrnS, and 0-0.7% for pnad5. The inter-specific sequence differences between H. nana and Hymenolepis diminuta were significantly higher, which was 31.6-31.7% (pnadS), 16.1-17.6% (wiS), and 26.5-27.1% (atp6). Phylogenetic analysis based on the combined three sequences using the maximum parsimony (MP) method supported that H. nana is a species complex or "cryptic" species. These findings demonstrated clearly the usefulness of the three mtDNA sequences for population genetics and systematic studies of H. nana of human and animal health significance.

分类号： Q7

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