In vitro fermentation behaviors of fucosylated chondroitin sulfate from Pearsonothuria graeffei by human gut microflora

文献类型： 外文期刊

第一作者： Wei, Chao-Yang

作者： Wei, Chao-Yang;Zhang, Yu;Ye, Xing-Qian;Li, Shan;Hu, Ya-Qin;Liu, Dong-Hong;Chen, Shi-Guo;Liao, Ning-Bo;Linhardt, Robert J.;Linhardt, Robert J.;Linhardt, Robert J.;Wang, Xin

作者机构：

关键词： Fucosylated chondroitin sulfate;Pearsonothuria graeffei;In vitro fermentantion;Intestinal microflora;Gut ecology

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

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收录情况： SCI

摘要： A fucosylated chondroitin sulfate (FCS-pg) with highly repeated structure from Pearsonothuria graeffei was subjected to a in vitro fermentation model to investigate its fermentability and effects on human gut microflora. High performance liquid chromatography (HPLC) measurement found FCS-pg can be fermented to short chain fatty acids (SCFAs) by gut microflora from partial human fecal samples. 16S rRNA gene-based polymerase chain reaction-based denaturing gradient gel electrophoresis (PCR-DGGE) profiling and real-time quantitative PCR analysis showed that FCS-pg mainly increased the proportions of Clostridium cluster XI, Bacteriodes prevotella group, Bifid bacterium genus, Clostridium cluster I and Clostridium cluster XlVab, whereas the numbers of the Enterobacteriaceae and Lactobacillus decreased. These results indicated that FCS-pg was mainly fermented by Bacteroides, Bifido bacterium and Clostridium. It increased the content of probiotics bacteria in achieving health-enhancing effect, was slightly different than most sulfated polysaccharides from marine animals. The current study provides useful new information on the mechanism of absorption and functional activity on FCS-pg within the gastrointestinal tract of the human body. (C) 2017 Published by Elsevier B.V.

分类号： Q71

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