Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates

文献类型： 外文期刊

第一作者： Yablonovitch, Arielle L.

作者： Yablonovitch, Arielle L.;Fu, Jeremy;Tang, Hua;Li, Jin Billy;Yablonovitch, Arielle L.;Li, Jin Billy;Li, Kexin;Rashkovetsky, Eugenia;Korol, Abraham B.;Nevo, Eviatar;Li, Kexin;Mahato, Simpla;Zelhof, Andrew C.;Kang, Lin;Michalak, Pawel;Michalak, Pawel;Michalak, Pawel

作者机构：

期刊名称：NATURE COMMUNICATIONS （ 影响因子：14.919； 五年影响因子：15.805 ）

ISSN： 2041-1723

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Determining the mechanisms by which a species adapts to its environment is a key endeavor in the study of evolution. In particular, relatively little is known about how transcriptional processes are fine-tuned to adjust to different environmental conditions. Here we study Drosophila melanogaster from 'Evolution Canyon' in Israel, which consists of two opposing slopes with divergent microclimates. We identify several hundred differentially expressed genes and dozens of differentially edited sites between flies from each slope, correlate these changes with genetic differences, and use CRISPR mutagenesis to validate that an intronic SNP in prominin regulates its editing levels. We also demonstrate that while temperature affects editing levels at more sites than genetic differences, genetically regulated sites tend to be less affected by temperature. This work shows the extent to which gene expression and RNA editing differ between flies from different microclimates, and provides insights into the regulation responsible for these differences.

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