Development of a serotype colloidal gold strip using monoclonal antibody for rapid detection type Asia1 foot-and-mouth disease
文献类型: 外文期刊
第一作者: Lin, Tong
作者: Lin, Tong;Shao, Jun-jun;Du, Jun-zheng;Cong, Guo-zheng;Gao, Shan-dian;Chang, Huiyun
作者机构:
关键词: Foot-and-Mouth disease virus (FMDV);type Asia1;Monoclonal antibodies;Colloidal gold strip;RIHA assay
期刊名称:VIROLOGY JOURNAL ( 影响因子:4.099; 五年影响因子:3.719 )
ISSN: 1743-422X
年卷期: 2011 年 8 卷
页码:
收录情况: SCI
摘要: Background: In this study, we developed a rapid, one step colloid gold strip (CGS) capable of specifically detecting type Asia1 foot-and-mouth disease virus (FMDV). We have produced two monoclonal antibodies (mAb) to type Asia1 FMD (named 1B8 and 5E2). On the test strip, the purified 1B8 labelled with the colloidal gold was used as the detector, and the purified 5E2 and goat anti-mouse antibodies were wrapped onto nitrocellulose (NC) membranes as the test and the control line, respectively. The rapid colloidal gold stereotype diagnostic strip was housed in a plastic case. Results: In specificity and sensitivity assay, there was no cross-reaction of the antigen with the other type of FMD and SVDV. The detection sensitivity was found to be as high as 10(-5) dilution of Asia1/JSL/05 (1 x 10(7.2)TCID(50)/50 mu L). There was excellent agreement between the results obtained by CGS and reverse indirect hemagglutination assay (RIHA), and the agreement can reach to 98.75%. Conclusion: We developed colloidal gold strips that have good qualities and does not require specialized equipment or technicians. This method provided a feasible, convenient, rapid, and effective for detecting type Asia1 FMDV in the fields.
分类号:
- 相关文献
作者其他论文 更多>>
-
A vesicular stomatitis virus-based African swine fever vaccine prototype effectively induced robust immune responses in mice following a single-dose immunization
作者:Ma, Yunyun;Shao, Junjun;Liu, Wei;Gao, Shandian;Peng, Decai;Miao, Chun;Yang, Sicheng;Hou, Zhuo;Zhou, Guangqing;Chang, Huiyun;Qi, Xuefeng
关键词:African swine fever virus; vaccine prototypes; vesicular stomatitis virus; safety; immune potency
-
Piriformospora indica Enhances Resistance to Fusarium wilt in Strawberry by Increasing the Activity of Superoxide Dismutase, Peroxidase, and Catalase, While Reducing the Content of Malondialdehyde in the Roots
作者:Huang, Yuji;Li, Jinman;Nong, Chaocui;Lin, Tong;Feng, Xu;Lin, Yuling;Lai, Zhongxiong;Miao, Lixiang;Huang, Yuji;Fang, Li;Chen, Yiting
关键词:strawberry; Piriformospora indica; Fusarium wilt; Benihoppe
-
Ultrasensitive detection assay for Cronobacter sakazakii based on nucleic acid-driven aggregation-induced emission of gold nanoclusters and cascaded signal amplification
作者:Kang, Qing;Zhang, Shuai-qi;Lin, Tong;Li, Jin-zhao;Ma, Chen-jing;Jiao, Jing -bo;Du, Xin-jun;Wang, Shuo;Li, Cheng
关键词:C. sakazakii; Gold nanoclusters; Signal amplification; Aggregation induced emission; G-quadruplex
-
A recombinant multi-epitope trivalent vaccine for foot-and-mouth disease virus serotype O in pigs
作者:Shao, Junjun;Liu, Wei;Gao, Shandian;Chang, Huiyun;Guo, Huichen
关键词:Foot-and-mouth disease virus (FMDV); Epitope; Vaccine; Immunization; Pig
-
Development of plate-type and tubular chemiluminescence immunoassay against African swine fever virus p72
作者:Miao, Chun;Shao, Junjun;Yang, Sicheng;Wen, Shenghui;Ma, Yunyun;Gao, Shandian;Chang, Huiyun;Liu, Wei;Miao, Chun;Shao, Junjun;Yang, Sicheng;Wen, Shenghui;Ma, Yunyun;Gao, Shandian;Chang, Huiyun;Liu, Wei
关键词:African swine fever virus; p72-CLIA; p72-MPCLIA; Diagnosis
-
Evaluation of humoral and cellular immune responses induced by a cocktail of recombinant African swine fever virus antigens fused with OprI in domestic pigs
作者:Zhang, Guanglei;Liu, Wei;Yang, Sicheng;Ma, Yunyun;Zhou, Guangqing;Liang, Xiaxia;Miao, Chun;Li, Junhui;Liu, Yanhong;Shao, Junjun;Chang, Huiyun;Song, Shuai;Zhang, Guanglei
关键词:African swine fever virus; Recombinant fusion proteins; OprI; Immune responses; Vaccine
-
Structure and function of African swine fever virus proteins: Current understanding
作者:Yang, Sicheng;Miao, Chun;Liu, Wei;Zhang, Guanglei;Shao, Junjun;Chang, Huiyun
关键词:African swine fever; African swine fever virus; structural proteins; non-structural proteins; capsid protein; inhibitor of apoptosis protein