Derivation and Characteristics of ES-Like Cells in Chicken Fetal Ovarian

文献类型: 外文期刊

第一作者: Zhang, Chuan-Sheng

作者: Zhang, Chuan-Sheng;Geng, Li-Ying;Yin, Chun-Guang;Li-Xin, D. U.

作者机构:

关键词: Embryonic stem cells;fetal chicken ovarian;in vitro culture;genome;comparison;China

期刊名称:JOURNAL OF ANIMAL AND VETERINARY ADVANCES ( 影响因子:0.365; 五年影响因子:0.359 )

ISSN: 1680-5593

年卷期: 2011 年 10 卷 2 期

页码:

收录情况: SCI

摘要: In the present study, the ES-like cells were derived from chicken fetal ovarian. These cells exhibit features similar to those of chicken EG cells such as typical bird nest morphology, strong reactivity toward periodic acid-Schiff and alkaline phosphatase staining. They could be maintained for more than a month on STO feeder in vitro, expressed the pluripotent markers such as stage specific embryonic antigen-1, stage-specific embryonic antigen-4, tumor rejection antigen-1-60 and tumor rejection antigen-1-81. Furthermore, the ES-like cells could form embryoid bodies and differentiate to several kinds of cells in vitro. Their. easy accessibility makes these cells an attractive alternative to chicken ES cells for future functional and genomic studies and comparison with other stem cells.

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