Culture of Chicken Embryonic Stem Cells in Buffalo Rat Liver 3A Cells (BRL-3A) Conditioned Medium
文献类型: 外文期刊
第一作者: Zhang, Chuan-Sheng
作者: Zhang, Chuan-Sheng;Geng, Li-Ying;Du, Li-Xin
作者机构:
关键词: Chicken;BRL-3A cells;in vitro culture;ES cell;embryonic stem;cell clones
期刊名称:JOURNAL OF ANIMAL AND VETERINARY ADVANCES ( 影响因子:0.365; 五年影响因子:0.359 )
ISSN: 1680-5593
年卷期: 2011 年 10 卷 6 期
页码:
收录情况: SCI
摘要: BRL-3A conditioned medium combined with basic Fibroblast Growth Factor (bFGF), Insulin-like Growth Factor (IGF), Stem Cell Factor (SCF) and STO cells are used for the culture of the feeder layer and ES (Embryonic Stem) cell clones. This study is done by isolation of chicken X-stage blastodermal cells. The putative ES cell clone features a typical bird's nest structure, PAS and AKP-positive and strongly positive for SSEA-1. It is able to form embryoid bodies and multiple types of cells as well as participates in embryonic development and feather color chimera formation through in vitro differentiation.
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