Detection of Different Genotypes of Clostridium perfringens in Feces of Healthy Dairy Cattle from China using Real-Time Duplex PCR Assay

文献类型： 外文期刊

第一作者： Qiu, Changqing

作者： Qiu, Changqing

作者机构：

关键词： Beta2-toxin;Clostridium perfringens;Duplex qPCR;Toxin typing

期刊名称：PAKISTAN VETERINARY JOURNAL （ 影响因子：1.318； 五年影响因子：0.954 ）

ISSN： 0253-8318

年卷期： 2011 年 31 卷 2 期

页码：

收录情况： SCI

摘要： Dual-labeled fluorescence hybridization probe-based multiplex quantitative real-time polymerase chain reaction (qPCR) assay was used for the detection of Clostridium perfringens toxin genes alpha (cpa), beta (cpb), iota (ia), epsilon (etx), beta2 (cpb2) and enterotoxin (cpe) directly from the feces of cattle. Fecal samples from 261 lactating cattle, belonging to three dairy herds in Ningxia (China), were examined using the developed assays. The duplex qPCR assay revealed that cpa, etx, cpb2 and cpe toxin genes were detected in 176 (100%), 15 (8.5%), 142 (80.7%) and 4 (2.3%) of 176 PCR positive samples, respectively. The findings of this study revealed that C. perfringens beta2-toxin-producing strains were widely prevalent in lactating cows in Ningxia, possibly playing an important role in C. perfringens-associated diarrheal disease. (C) 2011 PVJ. All rights reserved

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