Development of an indirect ELISA to specifically detect antibodies against African swine fever virus: bioinformatics approaches

文献类型： 外文期刊

第一作者： Gao, Zhan

作者： Gao, Zhan;Shao, Jun-Jun;Zhang, Guang-Lei;Ge, Su-Dan;Chang, Yan-Yan;Xiao, Lei;Chang, Hui-Yun

作者机构：

关键词： African swine fever virus; Indirect enzyme-linked immunosorbent assay; Recombinant protein; Multi-epitope

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN：

年卷期： 2021 年 18 卷 1 期

页码：

收录情况： SCI

摘要： Background African swine fever (ASF), characterized by acute, severe, and fast-spreading, is a highly lethal swine infectious disease caused by the African swine fever virus (ASFV), which has caused substantial economic losses to the pig industry worldwide in the past 100 years. Methods This study started with bioinformatics methods and verified the epitope fusion protein method's reliability that does not rely on traditional epitope identification. Meanwhile, it will also express and purify the constructed genes through prokaryotic expression and establish antibody detection methods. Results The results indicated that the protein had good reactivity and did not cross-react with other swine diseases. The receiver-operating characteristic analysis was performed to verify the determination. The area under the receiver-operating characteristic curve was 0.9991 (95% confidence interval 0.9973 to 1.001). Conclusions It was proved that the recombinant protein is feasible as a diagnostic antigen to distinguish ASFV and provides a new idea for ASFV antibody detection.

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