Recombinant PRRSV expressing porcine circovirus sequence reveals novel aspect of transcriptional control of porcine arterivirus
文献类型: 外文期刊
第一作者: Zheng, Haihong
作者: Zheng, Haihong;Sun, Zhi;Long, Jinxue;Lu, Jiaqi;Lv, Jian;Yuan, Shishan;Zheng, Haihong;Zhu, Xing-Quan
作者机构:
关键词: PRRSV;Novel subgenomic RNAs;Transcription-regulating sequence;Cryptic TRS;Porcine circovirus
期刊名称:VIRUS RESEARCH ( 影响因子:3.303; 五年影响因子:3.445 )
ISSN: 0168-1702
年卷期: 2010 年 148 卷 1-2 期
页码:
收录情况: SCI
摘要: Porcine reproductive and respiratory syndrome virus (PRRSV) expresses its genes via a set of nested subgenomic(sg) mRNAs. Such discontinuous transcription is unique yet poorly understood for arterivirus. The utilization of transcription-regulating sequence (TRS) remains a puzzle, as many TRS-like sequences exist in viral genome, yet only six or seven sg mRNAs were transcribed in arterivirus infected cells. To investigate the transcriptional control of the porcine arterivirus, a recombinant PRRSV infectious cDNA clone pCPV expressing the capsid gene of porcine circovirus 2 (PCV2) between PRRSV ORF1b and ORF2a was developed. The rescued recombinant viruses contained a range of disparate deletions of the inserted PCV2 sequence, yet two stable recombinant viruses containing 41 and 275 nt of foreign sequences were generated upon plaque purification and serial passages. Further analysis of the sg RNA2 profile revealed that an array of novel sg RNA species was generated in cells infected with the recombinant virus. One group was formed by utilizing the inserted PCV2 sequence as TRS; another group was generated from cryptic TRS-like PRRSV sequences located 19, 37 and 97 nt immediately downstream of the PRRSV ORF2 AUG. These results demonstrated that (I) the recombinant virus from direct insertion of foreign sequences was genetically unstable, while two recombinant PRRSVs containing foreign sequence of 41 or 275 nt in length, respectively, became stable upon plaque purification and further serial passages; (2) PRRSV can utilize foreign TRS-like sequence as transcriptional promoter; (3) the insertion of foreign sequence provoked the generation of novel subgenomic RNAs utilizing cryptic TRS-like sequences that remain non-functional in native PRRSV. (c) 2009 Elsevier B.V. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
AP2X-8 Is Important for Tachyzoite Growth and Bradyzoite Differentiation of Toxoplasma gondii
作者:Sun, Li-Xiu;Liu, Guo-Hua;Sun, Li-Xiu;Wang, Meng;Xie, Shi-Chen;Fu, Bao-Quan;Elsheikha, Hany M.;Zhu, Xing-Quan
关键词:
Toxoplasma gondii ; toxoplasmosis; AP2 transcription factors; AP2X-8; tachyzoite growth; bradyzoite differentiation -
Molecular prevalence and associated risk factors of Entamoeba spp. in donkeys in Shanxi Province, North China
作者:Zhang, Ze-Dong;Xiao, Han-Dan;Wang, Dong-Yang;Su, Nan;Liu, Xun-Zhi;Wang, Zi-Rui;Zhu, Xing-Quan;Zhang, Shuo;Gao, Wen-Wei;Zhu, Xing-Quan;Xie, Shi-Chen
关键词:Entamoeba spp.; Donkeys; Prevalence; Zoonotic parasites; Shanxi Province
-
The effects of selenium on the translation efficiency of selenoproteins GPX1, GPX4, and TXNRD1 in oxLDL-induced EA.hy926 cells
作者:Lu, Jiaqi;Meng, Jing;Lang, Yanzhi;Ran, Linwu;Rehman, Abdul;Yan, Yamei
关键词:Selenium; Oxidized low density lipoprotein (oxLDL); Selenoprotein; Ribosome profiling; Translational efficiency
-
Sample-to-answer Aggregation-Induced Emission Lateral Flow Immunoassay (ALFIA) for rapid and sensitive on-site detection of Toxoplasma gondii antibodies across multiple host species
作者:He, Yuan-Hui;Ji, Shusen;Wu, Ze-Xuan;Liang, Yao;Lv, Yi-Han;Zhu, Xing-Quan;Wang, Bin;Xie, Shi-Chen
关键词:Toxoplasma gondii ( T. gondii ); Double antigen sandwich principle; Aggregation-induced emission lateral flow; immunoassay (ALFIA); Sample-to-answer detection; Cross-species detection
-
AP2XII-9 is essential for parasite growth and suppresses bradyzoite differentiation in Toxoplasma gondii
作者:Wu, Xiao-Jing;Wang, Meng;Zhang, Nian-Zhang;Li, Ting-Ting;Gao, Jin;Sun, Li-Xiu;Wang, Jin-Lei;Wu, Xiao-Jing;Gao, Jin;Zhu, Xing-Quan
关键词:AP2XII-9; apicoplast inheritance; asynchronous division; bradyzoite differentiation; growth; membrane defect;
Toxoplasma gondii -
Anti-Toxoplasma gondii efficacy of beta, beta-dimethylacrylshikonin and isobutyrylshikonin in vitro and in vivo
作者:Guo, Hai-Ting;Wang, Lu;Xie, Shi-Chen;Zheng, Wen-Bin;Zhu, Xing-Quan;Guo, Hai-Ting;Li, Zhong-Yuan;Zhai, Bintao
关键词:
Toxoplasma gondii ; Beta, beta-dimethylacrylshikonin; Isobutyrylshikonin; Anti-infection; Metabolomics -
Toxoplasma gondii PPM3H regulates the parasite virulence and modulates host immune and inflammatory responses in mice
作者:He, Jun-Jun;Ma, Jun;Deng, Meng-Ling;Wang, Yi-Dan;Zhang, Yu-Cong;Zou, Feng-Cai;He, Jun-Jun;Elsheikha, Hany M.;Zhu, Xing-Quan
关键词:
Toxoplasma gondii ; PPM3H; PP2C phosphatases; parasite effector proteins; virulence; host-pathogen interaction