In vitro and in vivo evaluation of small interference RNA-mediated gynaecophoral canal protein silencing in Schistosoma japonicum

文献类型： 外文期刊

第一作者： Cheng, Guofeng

作者： Cheng, Guofeng;Fu, Zhiqiang;Lin, Jiaojiao;Cai, Youmin;Shi, Yi;Zhou, Yuancong;Jin, Youxin

作者机构：

关键词： gynaecophoral canal protein;pairing;RNA interference;Schistosoma japonicum;schistosomiasis

期刊名称：JOURNAL OF GENE MEDICINE （ 影响因子：4.565； 五年影响因子：3.257 ）

ISSN： 1099-498X

年卷期： 2009 年 11 卷 5 期

页码：

收录情况： SCI

摘要： Background Schistosomiasis causes liver and intestinal damage and can be very debilitating. The pairing of a male worm with a female worm residing in the gynaecophoral canal of male plays a critical role in the development of female parasite. Because the male specific gynaecophoral canal protein of Schistosoma japonicum (SjGCP) is found in significant quantities in the adult female worm after pairing, it could play an important role in parasite pairing. Methods in the present study, three small interfering (si)RNA duplexes targeting the SjGCP gene were designed, synthesized and the silencing effects were evaluated in vitro as well as in mice infected with S. japonicum in vivo. Results In vitro studies using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time RT-PCR revealed the reduction of SjGCP at the transcript level. Similarly, western blotting and immunofluorescence studies showed its reduction at the protein level after treatment of parasites with siRNAs. At a concentration of 200 nm, two siRNAs totally abolished the parasite pairing. To evaluate such a pairing inhibitory effect in vivo, mice infected with S. japonicum were treated with siRNA and both parasite pairing and burden were evaluated. In vivo tests confirmed the in vitro silencing effect of SjGCP siRNA and revealed that the systemic delivery of siRNA significantly inhibited early parasite pairing and the associated burden. Conclusions Our preliminary results demonstrated that the SjGCP plays an important role in pairing and subsequent development in S. japonicum, and its silencing might have potential as a therapeutic approach for controlling schistosomiasis. Copyright (C) 2009 John Wiley & Sons, Ltd.

分类号：

  • 相关文献

[1]Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing. Cheng, GF,Lin, JJ,Feng, XG,Fu, ZQ,Jin, YM,Yuan, CX,Zhou, YC,Cai, YM. 2005

[2]A specific PCR assay for the identification and differentiation of Schistosoma japonicum geographical isolates in mainland China based on analysis of mitochondrial genome sequences. Zhao, Guang-Hui,Li, Juan,Song, Hui-Qun,Li, Xiao-Yan,Zou, Feng-Cai,Zhu, Xing-Quan,Zhao, Guang-Hui,Zhao, Guang-Hui,Li, Juan,Song, Hui-Qun,Chen, Fen,Lin, Rui-Qing,Yuan, Zi-Guo,Weng, Ya-Biao,Li, Xiao-Yan,Zou, Feng-Cai,Zhu, Xing-Quan,Hu, Min,Zhu, Xing-Quan.

[3]Prevention of Schistosoma japonicum infection in mice with long-acting praziquantel implants. Lei, Lei,Guo, Shengrong,Cheng, Liang,Hou, Jingwen,Zhu, Chuangang,Shi, Yaojun,Jiang, Yan,Lin, Jiaojiao. 2012

[4]A specific indel marker for the Philippines Schistosoma japonicum revealed by analysis of mitochondrial genome sequences. Li, Juan,Li, Juan,Zhu, Xing-Quan,Chen, Fen,Sugiyama, Hiromu,Blair, David,Lin, Rui-Qing.

[5]Dose-dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome (Schistosoma japonicum) by RNA Interference. Cheng, GF,Lin, JJ,Shi, Y,Jin, YX,Fu, ZQ,Jin, YM,Zhou, YC,Cai, YM.

[6]Biotechnological advances in the diagnosis, species differentiation and phylogenetic analysis of Schistosoma spp.. Zhao, Guang-Hui,Li, Juan,Li, Xiao-Yan,Zhu, Xing-Quan,Zhao, Guang-Hui,Li, Juan,Lin, Rui-Qing,Blair, David,Li, Xiao-Yan,Elsheikha, Hany M.,Zou, Feng-Cai,Zhu, Xing-Quan. 2012

[7]Altered levels of circulating miRNAs are associated Schistosoma japonicum infection in mice. Zhu, Lihui,Dao, Jinwei,Du, Xiaoli,Li, Hao,Lu, Ke,Liu, Jinming,Cheng, Guofeng. 2015

[8]Successful outcome of an integrated strategy for the reduction of schistosomiasis transmission in an endemically complex area. Li, Shi-Zhu,Qian, Ying-Jun,Wang, Qiang,Zhou, Xiao-Nong,Yang, Kun,Zhang, Hua-Ming,Liu, Jun,Chen, Mu-Hua,Huang, Xi-Bao,Xu, Yin-Long. 2012

[9]Nested-PCR assay for detection of Schistosoma japonicum infection in domestic animals. Zhang, Xin,He, Chuan-Chuan,Liu, Jin-Ming,Li, Hao,Lu, Ke,Fu, Zhi-Qiang,Zhu, Chuan-Gang,Hong, Yang,Jin, Ya-Mei,Lin, Jiao-Jiao,Liu, Yi-Ping,Tong, Lai-Bao,Zhou, De-bao,Zha, Li,Lin, Jiao-Jiao. 2017

[10]MicroRNAs: Potentially important regulators for schistosome development and therapeutic targets against schistosomiasis. Cheng, Guofeng,Jin, Youxin,Jin, Youxin. 2012

[11]Role of microRNAs in schistosomes and schistosomiasis. Zhu, Lihui,Liu, Jinming,Cheng, Guofeng. 2014

[12]Changes in microRNA expression in response to Schistosoma japonicum infection. Hong, Y.,Fu, Z.,Cao, X.,Lin, J.,Cao, X.,Lin, J..

[13]Rapid detection and identification of four major Schistosoma species by high-resolution melt (HRM) analysis. Li, Juan,Zhu, Xing-Quan,Li, Juan,Zhao, Guang-Hui,Lin, RuiQing,Blair, David,Sugiyama, Hiromu.

[14]Molecular characterization of SjBIRP, another apoptosis inhibitor, from Schistosoma japonicum. Dao, Jinwei,Zhu, Lihui,Luo, Rong,Hu, Chao,Wang, Yuqing,Li, Hao,Lu, Ke,Liu, Jinming,Lin, Jiaojiao,Cheng, Guofeng,Dao, Jinwei,Cheng, Guofeng. 2014

[15]Cloning and expression of gynecophoral canal protein gene of Schistosoma japonicum (Chinese strain). Jin, YM,Lin, JJ,Feng, XG,Zhang, L,Wu, XF,Zhou, YC,Cai, YM. 2002

[16]Cloning and expression of actin gene of Schistosoma japonicum Chinese strain. Zhu, JG,Lin, JJ,Feng, XG,Wu, XF,Zhou, YC,Cai, YM. 2000

[17]Production and testing of Schistosoma japonicum candidate vaccine antigens in the natural ovine host. Taylor, MG,Huggins, MC,Shi, FH,Lin, JJ,Tian, E,Ye, P,Shen, W,Qian, CG,Lin, BF,Bickle, QD. 1998

[18]A novel colloidal gold immunochromatography assay strip for the diagnosis of schistosomiasis japonica in domestic animals. Xu, Rui,Feng, Jintao,Hong, Yang,Lv, Chao,Zhao, Dengyun,Lin, Jiaojiao,Lu, Ke,Li, Hao,Liu, Jinming,Cao, Xiaodan,Wang, Tao,Zai, Jinli,Wang, Zhaozhe,Jia, Bingguang,Han, Qian,Zhu, Chuangang,Lin, Jiaojiao. 2017

[19]Studies on the expression of the 28kD glutathione S-transferase gene from Schistosoma japonicum in the silkworm (Bombyx mori) larvae and insect cells. Tian, E,Yang, GZ,Cai, YM,Shi, FH,Wu, XF. 1997

[20]Analysis of early hepatic stage schistosomula gene expression by subtractive expressed sequence tags library. Wang, Xinzhi,Feng, XinGang,Fu, Zhiqiang,Jin, Yamei,Peng, Jinbiao,Lin, JiaoJiao,Gobert, Geoffrey N.. 2009

作者其他论文 更多>>

微信小程序