Association of polymorphisms in the intron 1 of duck prolactin with egg performance

文献类型： 外文期刊

第一作者： Li, Hui-Fang

作者： Li, Hui-Fang;Zhu, Wen-Qi;Chen, Kuan-Wei;Zhang, Tang-Jie;Song, Wei-Tao

作者机构：

关键词： PRL gene;PCR-RFLP;Gaoyou duck;double-yolked egg

期刊名称：TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES （ 影响因子：0.581； 五年影响因子：0.78 ）

ISSN： 1300-0128

年卷期： 2009 年 33 卷 3 期

页码：

收录情况： SCI

摘要： Gaoyou duck (Chinese indigenous breed) is famous for double-yolked egg. In the present study, SNP of prolactin (PRL) gene intron 1 of Gaoyou duck was detected by PCR-RFLP and DNA sequencing. The results indicated that there were 2 Dra I recognition sites in intron 1 of the PRL gene, but only 1 was of polymorphism. A T/C mutation at the position of the 1326 bp of the PRL gene was found by DNA sequencing, and resulted in 3 genotypes AA, AB, and BB. The frequencies of genotype BB and allele B were the highest. The association analysis between the polymorphism PRL gene intron 1 and egg performance was carried out. The least square analysis showed that the BB ducks had significant egg weight at the age of 30 weeks than AB ducks (P < 0.01), the double-yolk percentage of the AB ducks was much higher than that of BB ducks (P < 0.05) in double-yolk ducks, but there was not significant difference in egg number, longest clutch days, and the body weight at first egg among the three genotypes.

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