Functional analysis of helicase gene promoter and homologous region 3 enhancer in Bombyx mori nuclear polyhedrosis virus

文献类型： 外文期刊

第一作者： Xiao, QL

作者： Xiao, QL;Zhang, ZF;Yi, YZ;He, JL;Wu, XF

作者机构： Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China;Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China; Chinese Acad Agr Sci, Sericultural Res Inst, Minist Agr, Key Lab Silkworm Biotechnol, Zhenjiang 212018, Peoples R China

关键词： Bombyx mori nuclear polyhedrosis virus;helicase gene promoter;enhancer;transient expression

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 2020影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 0582-9879

年卷期： 2001 年 33 卷 5 期

页码：

收录情况： SCI

摘要： The promoter of the helicase gene, including 510 bp upstream of ATG, was cloned and sequenced, and was found that it had both early and late RNA initiation sites. The initiation codon ATG was deleted by using point mutation. Luciferase gene, as a reporter gene, was fused with the promoter region to construct the plsmid pBmhel510luc. When pBmhel510luc was transfected into Bm-5 and Sf-21 cell lines, the helicase gene promoter was recognized by cellular RNA polymerase and transactivated by viral factors. Baculovirus homologous regions (hrs) act as viral DNA replication start sites, which also have been shown to alter the rate of transcription for cis-linked promoters. BmNPV hr3 was cloned into a downstream site of luc gene, to study the effect of this enhancer on hel510 promoter activity. The transient expression in transfected insect cell lines and silkworm larvae indicated that hr3 could enhance the transcriptional level of hel510 promoter by about 7 000 and 1 000 fold, respectively.

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