Cryopreservation of apple in vitro shoot tips by the droplet freezing method

文献类型： 外文期刊

第一作者： Zhao, YH

作者： Zhao, YH;Wu, YJ;Engelmann, F;Zhou, MD;Chen, SY

作者机构：

关键词： apple;shoot tips;cryopreservation;droplet freezing method

期刊名称：CRYO-LETTERS （ 影响因子：1.066； 五年影响因子：1.0 ）

ISSN： 0143-2044

年卷期： 1999 年 20 卷 2 期

页码：

收录情况： SCI

摘要： Shoot tips sampled on in vitro plantlets of 5 apple cultivars were successfully cryopreserved using the droplet freezing method. After 3 weeks of cold-hardening at 5 degrees C, the shoot tips were precultured in liquid medium with 1.0% DMSO for 24 h at 5 degrees C, treated for 75 min. at room temperature with 0.3 M sucrose and 15% DMSO, placed in 5 mu l droplets of cryoprotectant medium containing 0.3 M sucrose and 15% DMSO on aluminium foil and cooled to -40 degrees C at 0.2 degrees C/min before immersion in liquid nitrogen. Recovery rates of cryopreserved shoot tips ranged between 70 and 92%, depending on the cultivar. Regrowth of frozen shoot tips was direct, without callus formation.

分类号：

  • 相关文献

[1]Cryopreservation of apple shoot tips by encapsulation-dehydration: Effect of preculture, dehydration and freezing procedure on shoot regeneration. Zhao, YH,Wu, YJ,Engelmann, F,Zhou, MD,Zhang, DM,Chen, SY. 1999

[2]Shoot tip culture and cryopreservation for eradication of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) from apple rootstocks 'M9' and 'M26'. Li, B. -Q.,Feng, C. -H.,Hu, L. -Y.,Wang, M. -R.,Wang, Q. -C.,Feng, C. -H..

[3]Cryopreservation of apple shoot tips: Importance of cryopreservation technique and of conditioning of donor plants. Wu, YJ,Engelmann, F,Zhao, YH,Zhou, MD,Chen, SY. 1999

[4]Cryopreservation of apple dormant buds and shoot tips. Wu, YJ,Zhao, YH,Engelmann, F,Zhou, MD,Zhang, DM,Chen, SY. 2001

[5]Culture of shoot tips from adventitious shoots can eradicate Apple stem pitting virus but fails in Apple stem grooving virus. Wang, Min-Rui,Li, Bai-Quan,Feng, Chao-Hong,Wang, Qiao-Chun,Feng, Chao-Hong.

[6]Cryopreservation of Jerusalem artichoke cultivars using an improved droplet-vitrification method. Zhang, Jin-Mei,Han, Li,Lu, Xin-Xiong,Xin, Xia,Yin, Guang-Kun,He, Juan-Juan,Chen, Xiao-Ling,Han, Li,Wang, Ling,Volk, Gayle M..

[7]Optimization of droplet-vitrification protocol for carnation genotypes and ultrastructural studies on shoot tips during cryopreservation. Zhang, Jin-Mei,Zhang, Xiao-Ning,Xin, Xia,Yin, Guang-Kun,He, Juan-Juan,Chen, Xiao-Ling,Zhang, Xiao-Ning,Xu, You-Ming,Zhang, Xiao-Ning.

[8]CRYOPRESERVATION OF IN VITRO-GROWN SHOOT TIPS OF CHINESE MEDICINAL PLANT Atractylodes macrocephala KOIDZ. USING A DROPLET-VITRIFICATION METHOD. Zhang, Jin-mei,Lu, Xin-xiong,Xin, Xia,Yin, Guang-kun,He, Juan-juan,Chen, Xiao-ling,Huang, Bin,Volk, Gayle M.. 2015

[9]Cryopreservation of kiwi shoot tips. Wu, YJ,Zhao, YH,Engelmann, R,Zhou, MD. 2001

[10]Genetic stability assessments of plantlets regenerated from cryopreserved in vitro cultured grape and kiwi shoot-tips using RAPD. Zhai, ZY,Wu, YJ,Engelmann, F,Chen, RZ,Zhao, YH. 2003

[11]Cryopreservation of sea perch (Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization. Ji, XS,Chen, SL,Tian, YS,Yu, GC,Sha, ZX,Xu, MY,Zhang, SC. 2004

[12]Optimization of Culture and Cryopreservation of Hand-Made Clone (HMC) Buffalo (Bubalus bubalis) Embryos. Yang, Chunyan,Pang, Chunying,Zheng, Haiying,Huang, Fenxiang,Wang, Jian,Shang, Jianghua,Yang, Bingzhuang,Liang, Xianwei. 2013

[13]Cryopreservation of Citrus anthers in the National Crop Genebank of China. Zhang, Jin-Mei,Lu, Xin-Xiong,Xin, Xia,Yin, Guang-Kun,He, Juan-Juan,Huang, Bin,Chen, Xiao-Ling,Huang, Bin,Jiang, Dong. 2017

[14]Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques. Li, Ping,Li, Zhi-Hua,Dzyuba, Boris,Hulak, Martin,Rodina, Marek,Linhart, Otomar,Li, Ping,Li, Zhi-Hua,Dzyuba, Boris. 2010

[15]PLANT-REGENERATION FROM RICE (ORYZA-SATIVA L.) EMBRYOGENIC SUSPENSION CELLS CRYOPRESERVED BY VITRIFICATION. HUANG, CN,WANG, JH,YAN, QS,ZHANG, XQ,YAN, QF. 1995

[16]Cryopreservation of the sperm of spotted halibut Verasper variegates (Pleuronectiformes, Pleuronectidae). Liu, XZ,Zhang, SC,Zhang, YZ,Xu, YJ. 2006

[17]Birth of calves expressing the enhanced green fluorescent protein after transfer of fresh or vitrified/thawed blastocysts produced by somatic cell nuclear transfer. Gong, GC,Dai, YP,Fan, BL,Zhu, HB,Zhu, S,Wang, HP,Wang, LL,Tang, B,Li, R,Wan, R,Liu, Y,Huang, YH,Zhang, L,Sun, XZ,Li, N.

[18]Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Li, P,Hanania, U,Sahar, N,Mawassi, M,Gafny, R,Sela, I,Tanne, E,Perl, A.

[19]Establishment and biological characteristics of Ujumqin sheep fibroblast line. Na, Ri Su,Zhao, Qian Jun,Jin, Da Peng,Su, Xiao Hua,Chen, Xian Wei,Guan, Wei Jun,Ma, Yue Hui,Jin, Da Peng,Su, Xiao Hua,Chen, Xian Wei.

[20]Transcriptome analysis and identification of induced genes in the response of Harmonia axyridis to cold hardiness. Tang, Bin,Liu, Xiao-Jun,Shi, Zuo-Kun,Shen, Qi-Da,Xu, Yan-Xia,Wang, Shi-Gui,Wang, Su,Zhang, Fan.

作者其他论文 更多>>

微信小程序