Cryopreservation of apple in vitro shoot tips by the droplet freezing method

文献类型: 外文期刊

第一作者: Zhao, YH

作者: Zhao, YH;Wu, YJ;Engelmann, F;Zhou, MD;Chen, SY

作者机构:

关键词: apple;shoot tips;cryopreservation;droplet freezing method

期刊名称:CRYO-LETTERS ( 影响因子:1.066; 五年影响因子:1.0 )

ISSN: 0143-2044

年卷期: 1999 年 20 卷 2 期

页码:

收录情况: SCI

摘要: Shoot tips sampled on in vitro plantlets of 5 apple cultivars were successfully cryopreserved using the droplet freezing method. After 3 weeks of cold-hardening at 5 degrees C, the shoot tips were precultured in liquid medium with 1.0% DMSO for 24 h at 5 degrees C, treated for 75 min. at room temperature with 0.3 M sucrose and 15% DMSO, placed in 5 mu l droplets of cryoprotectant medium containing 0.3 M sucrose and 15% DMSO on aluminium foil and cooled to -40 degrees C at 0.2 degrees C/min before immersion in liquid nitrogen. Recovery rates of cryopreserved shoot tips ranged between 70 and 92%, depending on the cultivar. Regrowth of frozen shoot tips was direct, without callus formation.

分类号:

  • 相关文献

[1]Shoot tip culture and cryopreservation for eradication of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) from apple rootstocks 'M9' and 'M26'. Li, B. -Q.,Feng, C. -H.,Hu, L. -Y.,Wang, M. -R.,Wang, Q. -C.,Feng, C. -H..

[2]Cryopreservation of apple shoot tips by encapsulation-dehydration: Effect of preculture, dehydration and freezing procedure on shoot regeneration. Zhao, YH,Wu, YJ,Engelmann, F,Zhou, MD,Zhang, DM,Chen, SY. 1999

[3]Cryopreservation of apple dormant buds and shoot tips. Wu, YJ,Zhao, YH,Engelmann, F,Zhou, MD,Zhang, DM,Chen, SY. 2001

[4]Cryopreservation of apple shoot tips: Importance of cryopreservation technique and of conditioning of donor plants. Wu, YJ,Engelmann, F,Zhao, YH,Zhou, MD,Chen, SY. 1999

[5]Culture of shoot tips from adventitious shoots can eradicate Apple stem pitting virus but fails in Apple stem grooving virus. Wang, Min-Rui,Li, Bai-Quan,Feng, Chao-Hong,Wang, Qiao-Chun,Feng, Chao-Hong.

[6]Optimization of droplet-vitrification protocol for carnation genotypes and ultrastructural studies on shoot tips during cryopreservation. Zhang, Jin-Mei,Zhang, Xiao-Ning,Xin, Xia,Yin, Guang-Kun,He, Juan-Juan,Chen, Xiao-Ling,Zhang, Xiao-Ning,Xu, You-Ming,Zhang, Xiao-Ning.

[7]CRYOPRESERVATION OF IN VITRO-GROWN SHOOT TIPS OF CHINESE MEDICINAL PLANT Atractylodes macrocephala KOIDZ. USING A DROPLET-VITRIFICATION METHOD. Zhang, Jin-mei,Lu, Xin-xiong,Xin, Xia,Yin, Guang-kun,He, Juan-juan,Chen, Xiao-ling,Huang, Bin,Volk, Gayle M.. 2015

[8]Cryopreservation of Jerusalem artichoke cultivars using an improved droplet-vitrification method. Zhang, Jin-Mei,Han, Li,Lu, Xin-Xiong,Xin, Xia,Yin, Guang-Kun,He, Juan-Juan,Chen, Xiao-Ling,Han, Li,Wang, Ling,Volk, Gayle M..

[9]Graphene Oxide Restricts Growth and Recrystallization of Ice Crystals. Geng, Hongya,Bai, Guoying,Song, Yanlin,Wang, Jianjun,Liu, Xing,Shi, Guosheng,Fang, Haiping,Liu, Xing,Shi, Guosheng,Fang, Haiping,Ma, Ji,Chen, Jingbo,Wu, Zhuangyuan.

[10]Sperm of the giant grouper: cryopreservation, physiological and morphological analysis and application in hybridizations with red-spotted grouper. Tian, Yongsheng,Jiang, Jing,Wang, Na,Qi, Wenshan,Liang, You,Chen, Songlin,Jiang, Jing,Qi, Wenshan,Yang, Chuanjun,Zhai, Jieming,Li, Bo,Chen, Youming.

[11]Sperm cryopreservation of sex-reversed seven-band grouper, Epinephelus septemfasciatus. Tian, Yongsheng,Qi, Wenshan,Jiang, Jing,Wang, Na,Wang, Di,Chen, Chao,Chen, Songlin,Qi, Wenshan,Jiang, Jing,Wang, Di,Zhai, Jieming.

[12]Effects of cryopreservation on the survival rate of the seven-band grouper (Epinephelus septemfasciatus) embryos. Tian, Yongsheng,Jiang, Jing,Song, Lini,Chen, Zhangfan,Wang, Na,Chen, Songlin,Jiang, Jing,Song, Lini,Zhai, Jieming,Liu, Jiangchun.

[13]Is transcription in sperm stationary or dynamic?. Ren, Xiaoxia,Chen, Xiaoli,Wang, Dong,Wang, Zhenling.

[14]A simple pollen collection, dehydration, and long-term storage method for litchi (Litchi chinensis Sonn.). Wang, Limin,Wu, Jiefang,Chen, Jiezhen,Fu, Danwen,Zhang, Chunyang,Cai, Changhe,Ou, Liangxi,Wang, Limin,Wu, Jiefang,Chen, Jiezhen,Fu, Danwen,Zhang, Chunyang,Cai, Changhe,Ou, Liangxi.

[15]An approach for long-term preservation of Blumeria graminis f. sp tritici. Gong, Shuangjun,Yang, Lijun,Xiang, Libo,Shi, Wenqi,Xue, Minfeng,Zeng, Fansong,Zhang, Xuejiang,Wang, Hua,Yu, Dazhao.

[16]Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Li, P,Hanania, U,Sahar, N,Mawassi, M,Gafny, R,Sela, I,Tanne, E,Perl, A.

[17]Vitrification-cryopreservation, an efficient method for eliminating Candidatus Liberobacter asiaticus, the citrus Huanglongbing pathogen, from in vitro adult shoot tips. Ding, Fang,Zhong, Yun,Cao, Qing,Yi, Ganjun,Ding, Fang,Jin, Shuangxia,Hong, Ni,Wang, Guoping,Ding, Fang,Zhong, Yun,Cao, Qing,Yi, Ganjun,Cao, Qing,Hong, Ni,Wang, Guoping. 2008

[18]EFFECTS OF CRYOPRESERVATION ON MOTILITY CHARACTERISTICS AND ENZYME ACTIVITY OF SPERM IN A CHINESE FISH, Nibea albiflora. Huang, Xiaorong,Zhuang, Ping,Zhang, Longzhen,Zhao, Feng,Liu, Jianyi,Feng, Guangpeng,Zhang, Tao. 2014

[19]Cryopreservation of the sperm of spotted halibut Verasper variegates (Pleuronectiformes, Pleuronectidae). Liu, XZ,Zhang, SC,Zhang, YZ,Xu, YJ. 2006

[20]Cryopreservation of in vitro cultured shoot tips of strawberry. Zhao, Mizhen,Wang, Zhuangwei,Su, Jiale,Qian, Yaming. 2006

作者其他论文 更多>>