Cryopreservation of apple shoot tips: Importance of cryopreservation technique and of conditioning of donor plants

文献类型： 外文期刊

第一作者： Wu, YJ

作者： Wu, YJ;Engelmann, F;Zhao, YH;Zhou, MD;Chen, SY

作者机构：

关键词： apple (Malus spp.);cryopreservation;shoot tips;two-step freezing;vitrification;encapsulation-dehydration;cold-hardening;shoot tip water content

期刊名称：CRYOLETTERS （ 影响因子：1.066； 五年影响因子：1.0 ）

ISSN： 0143-2044

年卷期： 1999 年 20 卷 2 期

页码：

收录情况： SCI

摘要： In this gaper, the efficiency of three techniques (two-step freezing, vitrification, encapsulation-dehydration) for freezing irt vitro shoot tips was compared and the effect of conditioning of mother-plants was investigated. Cold-hardening mother plants for 3 weeks at 5 degrees C improved regrowth rate and pattern of shoot tips whatever the cryopreservation technique employed. Increasing the time during which mother-plants were maintained on standard medium without subculture before sampling of apices decreased the water content of shoot tips from about 85-88% to 63-66% (fresh weight basis) and increased regrowth rate and pattern whatever the cryopreservation technique employed. The best results (up to 86% regrowth after cryopreservation without any callusing) were obtained with the encapsulation-dehydration technique. Using apices sampled on mother-plants which had not been subcultured for 26 weeks allowed to reduce the duration of the sucrose pregrowth treatment of encapsulated apices, thus simplifying the protocol. This simplified encapsulation-dehydration protocol was successfully applied to II out of the 12 cultivars tested.

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